There is an error in the third sentence of the Identification of small RNAs within bovine serum via RNA-seq section of the Results. The correct sentence is: On average, 54% mapped to tRNA, ~10% to other non-miRNA databases while 13% of reads could not be reliably mapped at all (Fig 3A).
There are errors in Table 1. The publisher apologizes for these errors. Please see the corrected Table 1 here.
Table 1. Differences and similarities in known bovine miRNAs detected between fresh sera (stored at -80°C and <1 year from time of collection) and biobanked sera (stored at -20°C for 10–15 years after collection) following small RNA sequencing.
Fresh serum & Biobanked serum overlap | Fresh serum specific | Biobank serum specific | ||
---|---|---|---|---|
miR-486 | miR-223 | miR-660 | miR-210 | miR-1307 |
miR-423-5p | miR-222 | miR-127 | miR-224 | miR-17-5p |
miR-22-3p | let-7i | miR-125b | miR-532 | miR-204 |
miR-92a | miR-130b | miR-99a-5p | miR-665 | miR-2285k |
miR-191 | miR-16a | miR-92b | miR-141 | miR-26b |
miR-140 | miR-2419-5p | miR-93 | miR-365-3p | miR-6119-5p |
miR-30d | miR-19b | miR-100 | miR-199a-3p | miR-98 |
miR-10b | miR-326 | miR-26a | miR-138 | let-7c |
miR-192 | miR-126-5p | miR-1468 | miR-193b | miR-28 |
miR-423-3p | miR-652 | miR-342 | miR-361 | miR-21-3p |
miR-16b | let-7g | 151-5p | miR-432 | |
miR-221 | miR-345-3p | miR-769 | miR-760-3p | |
miR-378 | let-7d | miR-197 | miR-130a | |
miR-151-3p | miR-30b-5p | miR-421 | miR-425-5p | |
miR-103 | miR-301a | let-7f | miR-133a | |
miR-21-5p | miR-181a | miR-23b-3p | ||
miR-107 | miR-320a | miR-15a | ||
miR-215 | miR-186 | let-7a-5p | ||
miR-30a-5p | miR-142-5p | miR-205 | ||
miR-2284x | miR-27b | miR-101 | ||
miR-296-3p | miR-30e-5p | miR-328 | ||
miR-29a | miR-148a | miR-126-3p | ||
miR-27a-3p | miR-150 | miR-330 | ||
miR-143 | miR-6529a | |||
miR-181b | miR-10a |
Reference
- 1.Shaughnessy RG, Farrell D, Riepema K, Bakker D, Gordon SV (2015) Analysis of Biobanked Serum from a Mycobacterium avium subsp paratuberculosis Bovine Infection Model Confirms the Remarkable Stability of Circulating miRNA Profiles and Defines a Bovine Serum miRNA Repertoire. PLoS ONE 10(12): e0145089 doi:10.1371/journal.pone.0145089 [DOI] [PMC free article] [PubMed] [Google Scholar]