Figure 8. Reduction of the SCAR-Arp complex activity suppresses the DC defects and ameliorates the loss of DE-cadherin in the AS of embryos expressing the foscrbY10A variant.
(A-F) Stills from dorsal views of live imaging of embryos expressing DE-cad::GFP knock-in and heterozygous for the SCAR∆37 loss of function allele (Video 13). In all images the anterior is to the left, for the genotypes w;foscrb,DE-cad::GFP/SCAR∆37,DE-cad::GFP;crbGX24 and w;foscrbY10A,DE-cad::GFP/SCAR∆37,DE-cad::GFP;crbGX24. All embryos were collected at the same time (1 hr collection), incubated at 28ºC for 7 hr and imaged together. The numbers in (B,D,F) indicate the time in minutes for the corresponding row. DC occurs normally in foscrb (A,C,D) embryos heterozygous for the SCAR∆37 allele, and DC defects are suppressed in foscrbY10A (B,D,F) embryos; some defects still visible include the impaired GB retraction (compare B with A), asymmetric position of the posterior spiracles (D, arrows), and bunching of the dorsal epidermis (D, bracket). Scale bar: 100 μm. (G,H) Magnified views of AS from (A,B, respectively). Note that, in order to make the localisation of DE-cad::GFP more perceptible, the autofluorescence of the yolk (visible in A,B) was removed from the original stack by hand using Fiji. Scale bar: 100 μm. Representative images from 6–9 different embryos for each genotype.