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. 1968 Nov;23(6):645–651. doi: 10.1136/thx.23.6.645

Perfusion and ventilation of isolated canine lungs

T J Otto 1,1, M Trenkner 1, A Stopczyk 1, M Gawdziński 1, B Chełstowska 1
PMCID: PMC471878  PMID: 4886091

Abstract

In order to evaluate methods of preserving lungs for use in transplantation, experiments on 28 mongrel dogs were carried out. Two methods were tried—first, mechanical respiration of isolated lungs under deep hypothermia, with the vascular bed filled with blood; and, secondly, the perfusion of isolated lungs with the aid of a modified DeWall's apparatus. Allogenic transplantations of lungs preserved in both ways were carried out. Gasometric and histological examinations of preserved lungs, before and after transplantation, were performed. The best results were obtained with perfusion under hypothermic conditions; ventilation without perfusion resulted in failure. Lung transplantation was successful when, after being preserved, the lung remained unchanged. Major discrepancies between the macroscopic and microscopic findings in preserved lungs were observed. An original classification of the changes occurring in preserved lungs is proposed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLUMENSTOCK D. A., HECHTMAN H. B., COLLINS J. A. Preservation of the canine lung. J Thorac Cardiovasc Surg. 1962 Dec;44:771–775. [PubMed] [Google Scholar]
  2. CAMPBELL G. S., CRISP N. W., BROWN E. B., Jr Total cardiac by-pass in humans utilizing a pump and heterologous lung oxygenator (dog lungs). Surgery. 1956 Aug;40(2):364–371. [PubMed] [Google Scholar]
  3. HARDY J. D., ERASLAN S., WEBB W. R. TRANSPLANTATION OF THE LUNG. Ann Surg. 1964 Sep;160:440–448. doi: 10.1097/00000658-196409000-00008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LILLEHEI C. W., DEWALL R. A. Design and clinical application of the Helix reservoir pump-oxygenator system for extracorporeal circulation. Postgrad Med. 1958 May;23(5):561–573. doi: 10.1080/00325481.1958.11692091. [DOI] [PubMed] [Google Scholar]
  5. Largiadèr F., Manax W. G., Lyons G. W., Lillehei R. C. Technical aspects of transplantation of preserved lungs. Dis Chest. 1966 Jan;49(1):1–7. doi: 10.1378/chest.49.1.1. [DOI] [PubMed] [Google Scholar]
  6. MARCHIORO T. L., HUNTLEY R. T., WADDELL W. R., STARZL T. E. EXTRACORPOREAL PERFUSION FOR OBTAINING POSTMORTEM HOMOGRAFTS. Surgery. 1963 Dec;54:900–911. [PMC free article] [PubMed] [Google Scholar]
  7. POTTS W. J., RIKER W. L., DeBORD R., ANDREWS C. E. Maintenance of life by homologous lungs and mechanical circulation. Surgery. 1952 Jan;31(1):161–166. [PubMed] [Google Scholar]
  8. Veith F. J., Deysine M., Nehlsen S. L., Karl R. C. Preservation of pulmonary function, hemodynamics, and morphology in isolated perfused canine lungs. J Thorac Cardiovasc Surg. 1966 Sep;52(3):437–441. [PubMed] [Google Scholar]
  9. Veith F. J., Hagstrom J. W., Nehlsen S. L., Karl R. C., Deysine M. Functional, hemodynamic, and anatomic changes in isolated perfused dog lungs: the importance of perfusate characteristics. Ann Surg. 1967 Feb;165(2):267–278. doi: 10.1097/00000658-196702000-00014. [DOI] [PMC free article] [PubMed] [Google Scholar]

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