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. 2016 Jan 7;5:e10147. doi: 10.7554/eLife.10147

Figure 3. Taxonomic distribution of proteins in the spindle orientation complex.

Figure 3.

(A) The genomes of the choanoflagellate Salpingoeca rosetta and the filasterean Capsaspora owczarzaki contain orthologs of human Dlg, Pins, and KHC-73. The domain architecture of each protein is shown, as inferred using the SMART database. Each group of proteins are reciprocal best BLAST hits (RBH) to the human query protein shown. For details, see Figure 3—source data 1. (B) Aligned sequences from the linker portion of Pins (see panel A), which binds to Dlg. Colors highlight identical or biochemically conservative residues. Asterisk, phosphorylated or negatively charged residue 436, which in the Drosophila melanogaster Pins protein anchors Dlg binding. For complete species names and accessions, see Figure 3—source data 2.

DOI: http://dx.doi.org/10.7554/eLife.10147.012

Figure 3—source data 1. Results of reciprocal Blast search of metazoan and nonmetazoan genomes for orthologs of GKPID, Pins and KHC-73.
Each row shows, for a query sequence from Drosophila melanogaster, the accession and e-score for the best-hit sequence in the target species, as well as the reciprocal best-hit and e-score when the target best-hit is used as a query against D. melanogaster.
DOI: http://dx.doi.org/10.7554/eLife.10147.013
elife-10147-fig3-data1.xlsx (10.9KB, xlsx)
DOI: 10.7554/eLife.10147.013
Figure 3—source data 2. Identifiers and species for Pins sequences shown in Figure 3B.
DOI: http://dx.doi.org/10.7554/eLife.10147.014
elife-10147-fig3-data2.xlsx (10.1KB, xlsx)
DOI: 10.7554/eLife.10147.014