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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Biochemistry. 2015 Dec 2;54(49):7248–7260. doi: 10.1021/acs.biochem.5b01082

Figure 1.

Figure 1

CheY-P dephosphorylation kinetics in the absence and presence of imidazole. (A) Phosphorimaging scans of SDS-PAGE gels showing the time-dependent loss of 32P from [32P]CheY-P in the absence (top panel) or presence (bottom panel) of 100 mM imidazole at pH 7.5. (B) Trp fluorescence time traces showing loss of phosphoryl groups with 0 (black), 50 mM (medium gray), or 500 mM (light gray) imidazole at pH 10.3.