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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Biochemistry. 2015 Dec 2;54(49):7248–7260. doi: 10.1021/acs.biochem.5b01082

Table 4.

Rate constants for “Asp → His” phosphotransfer

Receiver domain Amino acid at
yeast RR-P ➝ YPD1
CheY-P ➝ imidazole
D+2 T+1 T+2 kmaxa(s−1) Kda(μM) kmax/Kd (x 106 M−1s−1) kdephos,imidazole(M−1s−1)
SLN1/CheYSLN1 Q A F 29 ± 3 1.4 ± 0.6 21 8.2 ± 0.4
SSK1/CheYSSK1 Q A S -b - - 3.1 ± 0.1
SKN7/CheYSKN7 V G N 0.4 ± 0.1 5.0 ± 3.0 0.08 1.9 ± 0.4
a

Values are from 14.

b

Phosphotransfer was not observed from SSK1-P to YPD114.