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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Traffic. 2015 Dec 14;17(2):179–186. doi: 10.1111/tra.12347

Fig. 1.

Fig. 1

Simultaneous acquisition of multiple focal planes. (A) Schematic depicting how a 3D sample is imaged using a modified commercial Quad-view image splitter to generate a simultaneous 3D focal stack. (B) Example multifocus image of 500 nm fluorescent beads. The images have been aligned and intensity corrected. The relative approximate depth in z is indicated at the bottom of each image. Images are 18.5 μm × 18.5 μm. (C) Gaussian curves fitted to the bead signal of each plane at different z positions of the stage. (D) Plot of the best focus position of each plane.