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. 1965 Jul;18(4):446–452. doi: 10.1136/jcp.18.4.446

Studies on the serum iron-binding capacity

T K Stojceski 1,1, J S Malpas 1, L J Witts 1
PMCID: PMC472976  PMID: 14318697

Abstract

The total iron-binding capacity of serum has been estimated using a colorimetric method, a radioisotopic technique, and a method employing immunodiffusion. The modified isotopic technique described was considered to be the method of choice. The total iron-binding capacity starts to increase at an early stage of an iron-deficiency anaemia. It is decreased in infectious and malignant disease. Close correlation exists between the level of saturation of the total iron-binding capacity and the haemoglobin level in patients with uncomplicated iron-deficiency anaemia.

In patients with disease of the liver a difference was sometimes found between the total iron-binding capacity determined by the immunodiffusion method and by the two other techniques, the values with the immunodiffusion method being distinctly lower. This suggests the presence in the serum of iron which is not bound to transferrin. It is possible that ferritin was present in the serum in these cases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEUTLER E. Clinical evaluation of iron stores. N Engl J Med. 1957 Apr 11;256(15):692–697. doi: 10.1056/NEJM195704112561506. [DOI] [PubMed] [Google Scholar]
  2. BEUTLER E., ROBSON M. J., BUTTENWIESER E. A comparison of the plasma iron, iron-binding capacity, sternal marrow iron and other methods in the clinical evaluation of iron, stores. Ann Intern Med. 1958 Jan;48(1):60–82. doi: 10.7326/0003-4819-48-1-60. [DOI] [PubMed] [Google Scholar]
  3. BOTHWELL T. H., MALLETT B. The determination of iron in plasma or serum. Biochem J. 1955 Apr;59(4):599–602. doi: 10.1042/bj0590599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CONRAD M. E., Jr, BERMAN A., CROSBY W. H. Iron kinetics in laennec's cirrhosis. Gastroenterology. 1962 Oct;43:385–390. [PubMed] [Google Scholar]
  5. Cartwright G. E., Wintrobe M. M. CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. XXXIX. THE ANEMIA OF INFECTION. STUDIES ON THE IRON-BINDING CAPACITY OF SERUM. J Clin Invest. 1949 Jan;28(1):86–98. doi: 10.1172/JCI102058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FEINSTEIN A. R., BETHARD W. F., McCARTHY J. D. A new method, using radioiron, for determining the iron-binding capacity of human serum. J Lab Clin Med. 1953 Dec;42(6):907–914. [PubMed] [Google Scholar]
  7. GOODMAN M., NEWMAN H. S., RAMSEY D. S. The use of chicken antiserum for the rapid determination of plasma protein components. III. The assay of human serum transferrin. J Lab Clin Med. 1958 May;51(5):814–823. [PubMed] [Google Scholar]
  8. HATHORN M., GILLMAN T., CANHAM P. A., LAMONT N. M. Plasma iron and iron-binding capacity in African males with siderosis. Clin Sci. 1960 Feb;19:35–43. [PubMed] [Google Scholar]
  9. JAGER B. V., GUBLER C. J. An immunologic study of the iron-binding protein. J Immunol. 1952 Sep;69(3):311–318. [PubMed] [Google Scholar]
  10. KALDOR I. A shorter method for determining the latent iron-binding capacity of serum. Med J Aust. 1955 Nov 26;42(22):886–889. [PubMed] [Google Scholar]
  11. MITCHELL J., HALDEN E. R., JONES F., BRYAN S., STIRMAN J. A., MUIRHEAD E. E. Lowering of transferrin during iron absorption in iron deficiency. J Lab Clin Med. 1960 Oct;56:555–569. [PubMed] [Google Scholar]
  12. PETERS T., GIOVANNIELLO T. J., APT L., ROSS J. F. A new method for the determination of serum iron-binding capacity. I. J Lab Clin Med. 1956 Aug;48(2):274–279. [PubMed] [Google Scholar]
  13. RAMSAY W. N. The determination of the total iron-binding capacity of serum. Clin Chim Acta. 1957 Jun;2(3):221–226. doi: 10.1016/0009-8981(57)90106-7. [DOI] [PubMed] [Google Scholar]
  14. REISSMAN K. R., BOLEY J., CHRISTIANSON J. F., DELP M. H. The serum iron in experimental hepatocellular necrosis. J Lab Clin Med. 1954 Apr;43(4):572–582. [PubMed] [Google Scholar]
  15. REISSMANN K. R., DIETRICH M. R. On the presence of ferritin in the peripheral blood of patients with hepatocellular disease. J Clin Invest. 1956 Jun;35(6):588–595. doi: 10.1172/JCI103312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rath C. E., Finch C. A. CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. XXXVIII. SERUM IRON TRANSPORT. MEASUREMENT OF IRON-BINDING CAPACITY OF SERUM IN MAN. J Clin Invest. 1949 Jan;28(1):79–85. doi: 10.1172/JCI102057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SOOTHILL J. F. Estimation of eight serum proteins by a gel diffusion precipitin technique. J Lab Clin Med. 1962 May;59:859–870. [PubMed] [Google Scholar]
  18. VENTURA S. Determination of the unsaturated iron-binding capacity of serum. J Clin Pathol. 1952 Aug;5(3):271–274. doi: 10.1136/jcp.5.3.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. VERLOOP M. C., MEEUWISSEN J. E., BLOKHUIS E. W. Comparison of the iron absorption test with the determination of the iron-binding capacity of serum in the diagnosis of iron deficiency. Br J Haematol. 1958 Jan;4(1):70–81. doi: 10.1111/j.1365-2141.1958.tb03835.x. [DOI] [PubMed] [Google Scholar]
  20. WRIGHT S. T. A quantitative serum-agar technique. Nature. 1959 May 2;183(4670):1282–1283. doi: 10.1038/1831282b0. [DOI] [PubMed] [Google Scholar]

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