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. 1966 Jan;19(1):37–42. doi: 10.1136/jcp.19.1.37

Relative importance of formiminoglutamic and urocanic acid excretion after a histidine load

S D Mohamed 1,2, M Roberts 1,2
PMCID: PMC473155  PMID: 5904980

Abstract

A thin-layer chromatographic method for the simultaneous assessment of formimino-glutamic (Figlu) and urocanic acid excretion after loading with 15 g. histidine has been applied to 15 control subjects and to 49 selected patients. Upper limits of normal excretion were determined for urocanic acid and Figlu alone (15 mg. and 30 mg/8 hr. respectively), and for the combined metabolites (40 mg./8 hr.). Of the 49 patients studied, 27 excreted total metabolites above 40 mg./8 hr.; of these 19 would have been revealed by their abnormal excretion of Figlu and 22 by their abnormal excretion of urocanic acid. Of 74 tests in the 64 subjects, urocanic acid was present in all but 27% of the tests, and in 29·8% of the tests urocanic acid was in excess of the amount of Figlu excreted. In six normal and six abnormal tests urocanic acid was the sole metabolite present. These results re-emphasize the value of estimating both histidine metabolites in tests for folate deficiency in man, and underline the greater relative importance of urocanic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ACHESON R. M., PAUL R. M., TOMLINSON R. V. Some constituents of the urine of normal and schizophrenic individuals. Can J Biochem Physiol. 1958 Mar;36(3):295–305. [PubMed] [Google Scholar]
  2. BAKERMAN H. A., SILVERMAN M., DAFT F. S. Influence of succinylsulfathiazole and folic acid on glutamic acid excretion. J Biol Chem. 1951 Jan;188(1):117–123. [PubMed] [Google Scholar]
  3. BALDRIDGE R. C. The metabolism of histidine. II. Effects of folic acid deficiency. J Biol Chem. 1958 Mar;231(1):207–210. [PubMed] [Google Scholar]
  4. BENNETT M. C., CHANARIN I. Urinary excretion of urocanic acid and formimino-glutamic acid. Nature. 1962 Oct 20;196:271–272. doi: 10.1038/196271a0. [DOI] [PubMed] [Google Scholar]
  5. BOREK B. A., WAELSCH H. The enzymatic degradation of histidine. J Biol Chem. 1953 Nov;205(1):459–474. [PubMed] [Google Scholar]
  6. CAREY R. W., BRENA G. P., KRANT M. J. URINARY FORMIMINOGLUTAMIC ACID EXCRETION IN PATIENTS WITH NEOPLASTIC DISEASE. Cancer. 1964 Jun;17:713–722. doi: 10.1002/1097-0142(196406)17:6<713::aid-cncr2820170605>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
  7. CARTER F. C., HELLER P., SCHAFFNER G., KORN R. J. Formiminoglutamic acid (FIGLU) excretion in hepatic cirrhosis. Arch Intern Med. 1961 Jul;108:41–46. doi: 10.1001/archinte.1961.03620070043006. [DOI] [PubMed] [Google Scholar]
  8. CHANARIN I., MOLLIN D. L., ANDERSON B. B. Folic acid deficiency and the megaloblastic anaemias. Proc R Soc Med. 1958 Sep;51(9):757–763. [PMC free article] [PubMed] [Google Scholar]
  9. DELLER D. J., IBBOTSON R. N., CROMPTON B. METABOLIC EFFECTS OF PARTIAL GASTRECTOMY WITH SPECIAL REFERENCE TO CALCIUM AND FOLIC ACID. II. THE CONTRIBUTION OF FOLIC ACID DEFICIENCY TO THE ANAEMIA. Gut. 1964 Jun;5:225–229. doi: 10.1136/gut.5.3.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DYMOCK I. W. ABNORMAL URINARY EXCRETION OF UROCANIC ACID AND FORMIMINOGLUTAMIC ACID IN NEOPLASTIC DISEASE. Lancet. 1964 Jul 18;2(7351):114–115. doi: 10.1016/s0140-6736(64)90123-0. [DOI] [PubMed] [Google Scholar]
  11. GIRDWOOD R. H. Folic-acid-excretion studies in the investigation of malignant disease. Br Med J. 1953 Oct 3;2(4839):741–745. doi: 10.1136/bmj.2.4839.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HERBERT V., ZALUSKY R. Interrelations of vitamin B12 and folic acid metabolism: folic acid clearance studies. J Clin Invest. 1962 Jun;41:1263–1276. doi: 10.1172/JCI104589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KARLIN R. [On the value of the urinary folic acid excretion test for diagnosis of malignant disease]. Pathol Biol. 1963 Mar;11:289–295. [PubMed] [Google Scholar]
  14. KERSHAW P. W., GIRDWOOD R. H. SOME INVESTIGATIONS OF FOLIC-ACID DEFICIENCY. Scott Med J. 1964 May;9:201–212. doi: 10.1177/003693306400900503. [DOI] [PubMed] [Google Scholar]
  15. KNOWLES J. P., PRANKERD T. A. Abnormal folic acid metabolism in vitamin B12 deficiency. Clin Sci. 1962 Apr;22:233–238. [PubMed] [Google Scholar]
  16. LUHBY A. L., COOPERMAN J. M., TELLER D. N. Histidine metabolic loading test to distinguish folic acid deficiency from vit. B12 in megaloblastic anemias. Proc Soc Exp Biol Med. 1959 Jun;101(2):350–352. doi: 10.3181/00379727-101-24936. [DOI] [PubMed] [Google Scholar]
  17. LUHBY A. L., COOPERMAN J. M., TELLER D. N. Urinary excretion of formiminoglutamic acid: application in diagnosis of clinical folic acid deficiency. Am J Clin Nutr. 1959 Jul-Aug;7:397–406. doi: 10.1093/ajcn/7.4.397. [DOI] [PubMed] [Google Scholar]
  18. MOVITT E. R., GERSTL B., DAVIS A. E. Needle liver biopsy in thyrotoxicosis. AMA Arch Intern Med. 1953 Jun;91(6):729–739. doi: 10.1001/archinte.1953.00240180038005. [DOI] [PubMed] [Google Scholar]
  19. MYERS J. D., BRANNON E. S., HOLLAND B. C. A correlative study of the cardiac output and the hepatic circulation in hyperthyroidism. J Clin Invest. 1950 Aug;29(8):1069–1077. doi: 10.1172/JCI102338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. NORONHA J. M., SREENIVASAN A. Formate metabolism in the vitamin B12-deficient rat. Biochem J. 1959 Dec;73:732–735. doi: 10.1042/bj0730732. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SPRAY G. H., WITTS L. J. Excretion of formiminoglutamic acid as an index of folic-acid deficiency. Lancet. 1959 Oct 31;2(7105):702–704. doi: 10.1016/s0140-6736(59)92132-4. [DOI] [PubMed] [Google Scholar]
  22. SWENDSEID M. E., SWANSON A. L., MEYERS M. C., BETHELL F. H. The nutritional status of folic acid in persons with leukemia and its possible relation to effects of aminopterin therapy. Blood. 1952 Mar;7(3):307–310. [PubMed] [Google Scholar]
  23. VILLAMIL A., McCRACKEN B. H. Clinical usefulness of urinary Figlu test in megaloblastic anaemias. Br Med J. 1963 Mar 16;1(5332):717–720. doi: 10.1136/bmj.1.5332.717. [DOI] [PMC free article] [PubMed] [Google Scholar]

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