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The Journal of Biological Chemistry logoLink to The Journal of Biological Chemistry
. 2016 Jan 29;291(5):2547. doi: 10.1074/jbc.A110.216226

In TNF-stimulated cells, RIPK1 promotes cell survival by stabilizing TRAF2 and cIAP1, which limits induction of non-canonical NF-κB and activation of caspase-8.

Ian E Gentle, W Wei-Lynn Wong, Joseph M Evans, Alexandra Bankovacki, Wendy D Cook, Nufail R Khan, Ulrich Nachbur, James Rickard, Holly Anderton, Maryline Moulin, Josep Maria Lluis, Donia M Moujalled, John Silke, David L Vaux
PMCID: PMC4732234  PMID: 26826208

VOLUME 286 (2011) PAGES 13282–13291

There were several errors in this article.

PAGE 13284:

The figure legend of Fig. 1B incorrectly stated that 25 mg of protein was loaded. The correct amount is 25 μg. The description of Fig. 1C incorrectly stated that the concentration of chloroquine used was 200 mm. The correct concentration is 200 μm. The description of Fig. 1D applies to Fig. 1E, and the description for Fig. 1D should instead read “wild-type and three different RIPK1−/− MEF lines were treated with 100 ng/ml Fc-TNF for 0, 5, and 15 min. Cells were then lysed in DISC lysis buffer and split into soluble and insoluble fractions. 25 μg of protein from both fractions were run on SDS-PAGE and analyzed for levels of indicated proteins by Western blotting analysis.” An asterisk denotes a nonspecific band.

PAGE 13285:

In the figure legend for Fig. 2A, the text “Keratinocytes were isolated from day old RIPK−/− 1-day-old pups” does not refer to panel A of the figure and should be removed. The corrected text for the description of Fig. 2A should read, “E19 embryos were taken, and thymus was harvested. Thymi were dispersed into single cell suspensions, and half of each was left untreated, whereas the other half was treated with 100 ng/ml Fc-TNF for 1 h. Cells were lysed in DISC lysis buffer and analyzed for TRAF2 and cIAP1 by Western blotting analysis. Genotypes were confirmed by Western blotting analysis and PCR.”

PAGE 13286:

At the end of the figure legend for Fig. 4, the text “To control for NEC-1 activity, L929 cells” does not refer to this figure and should be removed.

PAGE 13287:

The following sentence should be added to the end of the legend to Fig. 6. “Error bars show S.E. of at least three independent experiments.”

PAGE 13288:

In Fig. 7A, the caspase-8 long exposure immunoblot was incorrectly cropped and the molecular mass of the β-actin immunoblot was incorrectly labeled. These errors have been corrected so that there are now six lanes in the caspase-8 (long exposure), and the molecular weight of actin reflects the actual size.

graphic file with name zbc0051638110001.jpg

The following sentence should be added to the figure legend to supplemental Fig. 2. “Error bars show S.E. of at least three independent experiments.”

These errors do not affect the results or conclusions of the work.


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