Abstract
Activator concentration in the range 50 to 100 Ploug units urokinase is directly proportional to the reciprocal of the lysis time both in the presence and absence of the plasma or serum inhibitors. Inhibitory activity can be estimated, therefore, by the difference in lysis time between control clots containing a buffer or euglobulin component and plasma or serum containing clots both activated by a common concentration of urokinase selected from within the critical range.
An experimental model for this purpose is described with an examination of the effects of each variable in this system and a reference curve in terms of a known inhibitor. In this study, it has been found that the effects of calcium are influenced by the plasminogen concentration and are biphasic in the presence of the serum inhibitors.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BRUCE S. THE EFFECT OF CALCIUM OF FIBRINOLYSIS IN VITRO. J Clin Pathol. 1964 May;17:282–286. doi: 10.1136/jcp.17.3.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FEARNLEY G. R., BALMFORTH G., FEARNLEY E. Evidence of a diurnal fibrinolytic rhythm; with a simple method of measuring natural fibrinolysis. Clin Sci. 1957 Nov;16(4):645–650. [PubMed] [Google Scholar]
- KOWALSKI E., KOPEC M., NIEWIAROWSKI An evaluation of the euglobulin method for the determination of fibrinolysis. J Clin Pathol. 1959 May;12(3):215–218. doi: 10.1136/jcp.12.3.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NILSSON I. M., KROOK H., STERNBY N. H., SODERBERG E., SODERSTROM N. Severe thrombotic disease in a young man with bone marrow and skeletal changes and with a high content of an inhibitor in the fibrinolytic system. Acta Med Scand. 1961 Mar;169:323–337. doi: 10.1111/j.0954-6820.1961.tb07838.x. [DOI] [PubMed] [Google Scholar]
- NORMAN P. S. Studies of the plasmin system. II. Inhibition of plasmin by serum or plasma. J Exp Med. 1958 Jul 1;108(1):53–68. doi: 10.1084/jem.108.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'CONNELL R. A., GROSSI C. E., ROUSSELOT L. M. ROLE OF INHIBITORS OF FIBRINOLYSIS IN HEPATIC CIRRHOSIS. Lancet. 1964 Nov 7;2(7367):990–991. doi: 10.1016/s0140-6736(64)90933-x. [DOI] [PubMed] [Google Scholar]
- PARASKEVAS M., NILSSON I. M., MARTINSSON G. A method for determining serum inhibitors of plasminogen activation. Scand J Clin Lab Invest. 1962;14:138–144. doi: 10.3109/00365516209079685. [DOI] [PubMed] [Google Scholar]
- SHERRY S., LINDEMEYER R. I., FLETCHER A. P., ALKJAERSIG N. Studies on enhanced fibrinolytic activity in man. J Clin Invest. 1959 May;38(5):810–822. doi: 10.1172/JCI103863. [DOI] [PMC free article] [PubMed] [Google Scholar]