Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1966 Sep;19(5):491–495. doi: 10.1136/jcp.19.5.491

A distinctive fraction of alkaline phosphatase in health and disease

M A Newton 1
PMCID: PMC473357  PMID: 5919363

Abstract

An electrophoretic fraction of plasma alkaline phosphatase, which migrates more slowly than the main fraction, was present in one fifth of normal subjects, and in some patients with parenchymal liver disease. It was absent in patients with bone disease, uncomplicated biliary obstruction, and hepatic metastases.

The electrophoretic and inhibition properties of this slow band were similar to those of intestinal phosphatase, and its significance is discussed.

In a patient with hypophosphatasia this fraction was apparently not decreased.

Full text

PDF
491

Images in this article

492Image
on p.492
493Image
on p.493
493Image
on p.493

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARFORS K. E., BECKMAN L., LUNDIN L. G. GENETIC VARIATIONS OF HUMAN SERUM PHOSPHATASES. Acta Genet Stat Med. 1963;13:89–94. doi: 10.1159/000151789. [DOI] [PubMed] [Google Scholar]
  2. BAMFORD K. F., HARRIS H., LUFFMAN J. E., ROBSON E. B., CLEGHORN T. E. SERUM-ALKALINE-PHOSPHATASE AND THE ABO BLOOD-GROUPS. Lancet. 1965 Mar 6;1(7384):530–531. doi: 10.1016/s0140-6736(65)92027-1. [DOI] [PubMed] [Google Scholar]
  3. BARRETT A. M., FAIRWEATHER D. V., MCCANCE R. A., MORRISON A. B. Genetic, clinical, biochemical, and pathological features of hypophosphatasia; based on the study of a family. Q J Med. 1956 Oct;25(100):523–537. [PubMed] [Google Scholar]
  4. BECKMAN L. ASSOCIATIONS BETWEEN HUMAN SERUM ALKALINE PHOSPHATASES AND BLOOD GROUPS. Acta Genet Stat Med. 1964;14:286–297. [PubMed] [Google Scholar]
  5. BETHUNE J. E., DENT C. E. Hypophosphatasia in the adult. Am J Med. 1960 Apr;28:615–622. doi: 10.1016/0002-9343(60)90156-x. [DOI] [PubMed] [Google Scholar]
  6. Boyer S. H. Alkaline Phosphatase in Human Sera and Placentae: Starch gel electrophoresis reveals many phosphatase components including a polymorphism in placentae. Science. 1961 Oct 6;134(3484):1002–1004. doi: 10.1126/science.134.3484.1002. [DOI] [PubMed] [Google Scholar]
  7. CHIANDUSSI L., GREENE S. F., SHERLOCK S. Serum alkaline phosphatase fractions in hepato-biliary and bone diseases. Clin Sci. 1962 Jun;22:425–434. [PubMed] [Google Scholar]
  8. Delory G. E. A sodium carbonate-bicarbonate buffer for alkaline phosphatases. Biochem J. 1945;39(3):245–245. [PMC free article] [PubMed] [Google Scholar]
  9. ESTBORN B. Visualization of acid and alkaline phosphatase after starch-gel electrophoresis of seminal plasma, serum and bile. Nature. 1959 Nov 21;184(Suppl 21):1636–1637. doi: 10.1038/1841636a0. [DOI] [PubMed] [Google Scholar]
  10. FAHEY J. L., McCOY P. F., GOULIAN M. Chromatography of serum proteins in normal and pathologic sera: the distribution of protein-bound carbohydrate and cholesterol, siderophilin, thyroxin-binding protein, B12-binding protein, alkaline and acid phosphatases, radio-iodinated albumin and myeloma proteins. J Clin Invest. 1958 Feb;37(2):272–284. doi: 10.1172/JCI103606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FISHMAN W. H., GREEN S., INGLIS N. I. Organ-specific behavior exhibited by rat intestine and liver alkaline phosphatase. Biochim Biophys Acta. 1962 Aug 13;62:363–375. doi: 10.1016/0006-3002(62)90266-4. [DOI] [PubMed] [Google Scholar]
  12. FRASER D. Hypophosphatasia. Am J Med. 1957 May;22(5):730–746. doi: 10.1016/0002-9343(57)90124-9. [DOI] [PubMed] [Google Scholar]
  13. GOYER R. A. Ethanolamine phosphate excretion in a family with hypophosphatasia. Arch Dis Child. 1963 Jun;38:205–207. doi: 10.1136/adc.38.199.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. GUTMAN A. B. Serum alkaline phosphatase activity in diseases of the skeletal and hepatobiliary systems. A consideration of the current status. Am J Med. 1959 Dec;27:875–901. doi: 10.1016/0002-9343(59)90173-1. [DOI] [PubMed] [Google Scholar]
  15. HODSON A. W., LATNER A. L., RAINE L. Iso-enzymes of alkaline phosphatase. Clin Chim Acta. 1962 Mar;7:255–261. doi: 10.1016/0009-8981(62)90018-9. [DOI] [PubMed] [Google Scholar]
  16. KEIDING N. R. Differentiation into three fractions of the serum alkaline phosphatase and the behavior of the fractions in diseases of bone and liver. Scand J Clin Lab Invest. 1959;11:106–112. doi: 10.3109/00365515909060415. [DOI] [PubMed] [Google Scholar]
  17. LeVEEN H. H., TALBOT L. J., RESTUCCIA M., BARBERIO J. R. Metabolism and excretion of alkaline phosphatase: relation to liver function and determination of maximal secretory rates of liver. J Lab Clin Med. 1950 Aug;36(2):192–205. [PubMed] [Google Scholar]
  18. MADSEN N. B., TUBA J. On the source of the alkaline phosphatase in rat serum. J Biol Chem. 1952 Apr;195(2):741–750. [PubMed] [Google Scholar]
  19. MORTON R. K. Separation and purification of enzymes associated with insoluble particles. Nature. 1950 Dec 30;166(4235):1092–1095. doi: 10.1038/1661092a0. [DOI] [PubMed] [Google Scholar]
  20. POULIK M. D. Starch gel electrophoresis in a discontinous system of buffers. Nature. 1957 Dec 28;180(4600):1477–1479. doi: 10.1038/1801477a0. [DOI] [PubMed] [Google Scholar]
  21. ROSENBERG I. N. Zone electrophoretic studies of serum alkaline phosphatase. J Clin Invest. 1959 Apr;38(4):630–644. doi: 10.1172/JCI103841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SCHLAMOWITZ M., BODANSKY O. Tissue sources of human serum alkaline phosphatase, as determined by immunochemical procedures. J Biol Chem. 1959 Jun;234(6):1433–1437. [PubMed] [Google Scholar]
  23. SHREFFLER D. C. GENETIC STUDIES OF BLOOD GROUP-ASSOCIATED VARIATIONS IN A HUMAN SERUM ALKALINE PHOSPHATASE. Am J Hum Genet. 1965 Jan;17:71–86. [PMC free article] [PubMed] [Google Scholar]
  24. SMITHIES O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J. 1955 Dec;61(4):629–641. doi: 10.1042/bj0610629. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES