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. 1966 Nov;19(6):617–618. doi: 10.1136/jcp.19.6.617

Effects of Triton-X-100 upon the mobility of esterases and alkaline phosphatases in disc electrophoresis

H B Coutinho 1,1, E Katchburian 1,2, A G E Pearse 1
PMCID: PMC473395  PMID: 5928611

Abstract

Homogenates of the mid gut and digestive glands of Rhynchosciara angelae were prepared in 40% sucrose, with and without 0·5% Triton-X-100. These were subjected to disc electrophoresis and zymograms for esterases and alkaline phosphatase were prepared. In both groups the effect of Triton-X-100 was to increase the electrophoretic mobility of the enzymes concerned.

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Selected References

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