Abstract
Mesenchymal stem cells (MSCs) possess self-proliferation and multi-directional differentiation abilities. Previous studies on MSCs have mostly focused on the bone marrow, lungs, pancreas and umbilical cord blood, with few studies on metanephric tissues in ducks. For the present study, the Beijing duck was selected as an experimental animal. Duck embryo metanephric mesenchymal stem cells (MMSCs) were studied. MMSC isolation culture, analysis of biological characteristics, induced differentiation and identification were performed in preliminary experiments. In the current study, surface antigens and gene expression patterns were detected using immunofluorescence, reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry. The induced cells, adipocytes, hepatocytes, epithelial cells and islet cells were identified by oil red O staining, periodic acid-Schiff staining, immunofluorescence and dithizone staining, respectively. RT-PCR was performed for detection of specific marker genes. The results suggested that the biological characteristics of MMSCs were similar to those of the MSCs previously analyzed. Primary MMSCs were sub-cultured to passage 21. The induced cells exhibit typical staining and immunofluorescence indicating the expression of specific genes. This demonstrates that MMSCs may be a novel alternative source of MSCs for experimental and clinical applications.
Keywords: Beijing duck, metanephric mesenchymal stem cells, differentiation
Introduction
Mesenchymal stem cells (MSCs) are multipotent cells which can proliferate and differentiate into numerous cell types, including adipocytes, hepatocytes, epithelial cells and islet cells (1). With rapid cell proliferation, low immunogenicity and the potential for straightforward transfection of foreign genes, MSCs have been extensively used as seed cells for tissue engineering (2). Previously, studies have demonstrated that MSCs can be transplanted to repair damaged tissues, with the potential for more valuable therapeutic research and clinical treatments to be conducted through investigating the biological characteristics of MSCs (3).
In the current study, the expression of a number of genes and proteins, including vimentin, fibronectin, CD73, CD71, CD44, CD34, CD29, CD45, Pax2 and CD166, were investigated in Beijing duck MMSCs. CD29 is an integrin unit associated with late antigen receptors, which can form a heterodimer with surface and extracellular proteins to mediate cell-cell and cell-matrix interactions (4). CD44 is a cell surface glycoprotein, known as an adhesion molecule. It can bind to collagen type I and fibronectin, and provide growth-anchoring sites for MSCs. CD71 is a member of the transferrin receptor family that is required for the import of iron into cells and is regulated in response to intracellular iron concentrations. In the event of low cellular iron concentration, the levels of transferrin receptors increase, thus causing an increase in the uptake of iron by the cells. Therefore, the transferrin receptor maintains cellular iron homeostasis (5).
Although metanephric mesenchymal stem cells (MMSCs) from humans, rats and livestock have been obtained and characterized, there are few reports of harvesting duck MMSCs (6). The Beijing duck is a domesticated Aves Anseriformes Anatidae (from the mallard duck species, Anas platyrhynchos), with a stable hereditary character and excellent fertility. The Beijing duck embryo metanephron can be obtained in a convenient, economic way without ethical or histocompatibility problems, or immune rejection (7). MMSCs have broad preclinical application prospects, such as cell transplant or tissue engineering (8).
In the present study, the self-renewal and differentiation capabilities and gene expression patterns in Beijing duck MMSCs were analyzed through in vitro cell culture for the first time, to the best of our knowledge.
Materials and methods
Experimental animals
All animal procedures were approved by the Institutional Animal Care and Use Committee of The Chinese Academy of Agricultural Sciences (Beijing, China). In total, 300 Beijing duck embryos (20 day-old) were provided by the Animal Husbandry Experimental Base Institute of Animal Sciences, Chinese Academy of Agricultural Sciences.
Isolation and culture of MMSCs
Enzymatic digestion was used as a stable method to harvest MMSCs from metanephric tissues. Initially, metanephros cells were collected from 20-day-old Beijing duck embryos. The duck metanephros were exposed and ureteric buds were removed subsequent to washing with phosphate-buffered saline (PBS; Sigma-Aldrich, Santa Clara, CA, USA). Tissue blocks were cut into 1-mm3 pieces and digested with 0.1% collagenase type IV (Sigma-Aldrich) for 25 min at 37°C, then neutralized with equal DMEM/F-12 containing 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, Inc., New York, NY, USA). The cell suspension was filtered through a 300 mesh stainless steel sieve and centrifuged at 250 × g for 8 min, then added to complete medium [DMEM/F-12, 10% FBS, 10 ng/ml leukemia inhibitory factor (LIF; Peprotech, Rocky Hill, NJ, USA)] and seeded into plates, incubated at 37°C with 5% CO2 (9). The non-adherent cells and fragments were removed with PBS 24 h post-seeding. When cells reached 80% confluence, 0.125% trypsin and 0.02% EDTA (Sigma-Aldrich) were added for subculturing. Purified MMSCs were obtained after 3 passages (10).
MTS cell viability assay
P5 generation cells were inoculated into 96-well plates at a cell density of 1.0×104 cells/ml. Following the treatment period, the cytotoxicity assay was performed using MTS reagent [3-(4, 5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium, inner salt] according to the manufacturer's protocol (Promega Corp., Beijing, China). Cell absorbance was spectrophotometrically measured using an ELx800 absorbance microplate reader (BioTek Instruments, Inc., Winooski, VT, USA) at 490 nm (11). A growth curve was produced using the average cell count data for each day of the 7-day study (12).
RNA extraction and reverse transcription-polymerase chain reaction (RT-PCR)
RNA was extracted from cells using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and cDNA was synthesized using an RNA PCR kit (Takara Biotechnology Co., Ltd., Dalian, China) (13). The cDNA was amplified by PCR with specific primers (designed by Sangon Biotech, Shanghai, China; Table I), using a Platinum PCR SuperMix (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. PCR was performed in a 20 µl solution containing 2.0 µl 10X RT buffer, 13.4 µl double-distilled H2O, 0.2 µl Ex-Taq (Takara Bio Inc., Otsu, Japan), 1.0 µl each of forward and reverse primers, 1.0 µl template cDNA and 1.4 µl dNTP (2.5 mM). The reaction conditions consisted of an initial denaturation step at 94°C for 5 min, followed by 30 cycles at 94°C for 30 sec, 55–60°C for 30 sec and 72°C for 30 sec, and a final cycle at 72°C for 10 min. The PCR products were visualized by 2.5% agarose gel electrophoresis (Gibco; Thermo Fisher Scientific, Inc.) at 140 V for 30 min (14).
Table I.
Primer sequences for reverse transcription-polymerase chain reaction.
| Gene name | Primer sequence | Tm (°C) | Product length (bp) |
|---|---|---|---|
| Fibronectin | F: 5′-CCTGTGTTCTGCCTTTCACC-3′ | 58 | 251 |
| R: 5′-GTTGTCTCTCCGTCCCTCAG-3′ | |||
| CD71 | F: 5′-GCTCGTGTGAATCCTGAACC-3′ | 58 | 290 |
| R: 5′-TATTGGAGGGCTGCTGTTG-3′ | |||
| CD34 | F: 5′-CTCAACGAGTCCAACACCTG-3′ | 60 | 338 |
| R: 5′-CCAGAAGTGACCAAAGCAGTC-3′ | |||
| CD29 | F: 5′-CACTCCCGTGCTGTGAATC-3′ | 60 | 257 |
| R: 5′-ACGCTGCTCATTTCCAACTC-3′ | |||
| GAPDH | F: 5′-GCACTGAACGACCATTTCG-3′ | 58 | 256 |
| R: 5′-CAGGTGGAGGAAGAAGTTGG-3′ | |||
| PPAR-γ | F: 5′-GCAGGAGATCACAGAATTTGA-3′ | 58 | 356 |
| R: 5′-TTGGGCTCCATAAAGTCACA-3′ | |||
| LPL | F: 5′-AGCTCTGAGTCTGATTGCTG-3′ | 58 | 256 |
| R: 5′-AATGGCTGGTTGGTCTTGGT-3′ | |||
| AFP | F: 5′-AACGATTGCTTTCTCTCCCTTA-3′ | 58 | 283 |
| R: 5′-TCACTACCTTTGGTGCCTGTC-3′ | |||
| ALB | F: 5′-GGCAAGGAAACTGGCATAAG-3′ | 58 | 317 |
| R: 5′-TCCACAATGGGCTTCTCAC-3′ | |||
| E-cadherin | F: 5′-TGCCACCAGTCAAGAAAGTG-3′ | 60 | 252 |
| R: 5′-ACCATTATCAACAGCCACGA-3′ | |||
| CK19 | F: 5′-ATCCTTGCTGCCACTATCG-3′ | 58 | 251 |
| R: 5′-GCACTCATTTCCTCCTCGTG-3′ | |||
| CD45 | F: 5′-CTCACCACACGCACTCTCAC-3′ | 60 | 350 |
| R: 5′-CTCTTCCCATCTTCCAGCAG-3′ | |||
| Pax2 | F: 5′-ATCTGCGACAACGACACG-3′ | 60 | 262 |
| R: 5′-CCTCGGACACATCTTCATCA-3′ | |||
| CD166 | F: 5′-AAGAAGACCTGCGTAACTGGA-3′ | 60 | 295 |
| R: 5′-CCTGCTAATGCCACGAGAGT-3′ | |||
| PDX-1 | F: 5′-GCTAATGAATACCGCAGACAGA-3′ | 58 | 314 |
| R: 5′-GAAGCAAAGGTTGGAATAGGC-3′ | |||
| Insulin | F: 5′-TGGAAGTGCGAAAGACACAC-3′ | 58 | 303 |
| R: 5′-GGTGAAAGGCAGAACACAGG-3′ |
F, forward; R, reverse.
Immunofluorescence analysis of MMSC surface antigens
Cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 20 min at room temperature and washed three times (every 5 min), permeabilized by 0.25% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) for 10 min, which was diluted with PBS (1:10), blocked with goat serum (OriGene Technologies, Beijing, China) for 60 min (15). The following antibodies were added: Rabbit anti-chicken antibodies against fibronectin, CD71 and CD73 (dilution, 1:100; cat. nos. bs-4859R, bs-1782R and bs-4834R, respectively; Beijing Biosynthesis Biotechnology Co. Ltd., Beijing, China), and mouse anti-chicken antibodies against CD29 and CD45 (dilution, 1:100; cat. nos. ab26841 and ab24909, respectively; Abcam, Cambridge, CA, USA). The samples were incubated with the antibodies overnight at 4°C. The primary antibody was discarded and washed, then the fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies (dilution, 1:100; cat. no. ZF-0311; OriGene Technologies) were added. The cells were then placed in the dark for 1 h at room temperature (16). The plates were washed and nuclear staining was performed with 1 µg/ml DAPI (Sigma-Aldrich) for 30 min (17). Immunofluorescence images were acquired using a laser-scanning confocal microscope (FV1000; Olympus Corp., Tokyo, Japan). Non-overlapping fields were observed and images were captured.
Flow cytometry analysis of MMSCs
The flow cytometry protocol used was similar to the immunofluorescence protocol described earlier, in terms of the cell preparation and treatment. However, the selected polyclonal antibodies used were as follows: Anti-vimentin, anti-CD44 and anti-CD71 antibodies (all rabbit anti-chicken antibodies; dilution, 1:100; cat. nos. bs-0756R, bs-2507R and bs-1782R, respectively; Beijing Biosynthesis Biotechnology Co. Ltd.). Flow cytometric analysis was performed on the mononuclear cell suspension, and 5×104 cells were incubated with 10 µl FITC-conjugated antibodies (18). Cells were acquired using a FACSCalibur flow cytometer and CytExpert Cell Quest software, and then analyzed with Paint-A-Gate software (BD Biosciences, San Jose, CA, USA).
Frozen storage and recovery
For freeze storage, 50% DMEM/F-12, 10% dimethyl sulfoxide and 40% FBS were used, and for long-term storage a liquid nitrogen tank (19) was used to preserve the genetic resources of the Beijing duck.
Differentiation of MMSCs
MMSCs with high reproductive ability were targeted for differentiation. When cell proliferation attained 50% confluence, cells were randomly divided into induced and control groups. The complete medium was used for the control group.
The induction medium for adipocyte differentiation consisted DMEM/F-12, 10% FBS, 10-7M dexamethasone, 8 µg/ml insulin, 70 µM indomethacin (Sigma-Aldrich), 0.5 mM 3-1-methyl isobutyl-xanthine (IBMX) and 1% glutamine supplement (Gibco; Thermo Fisher Scientific, Inc.) The induction was conducted for 6, 9 and 12 days. Accumulated oil droplets were detected by oil red O staining (Peprotech) (20).
Hepatocyte differentiation was induced using the following media: DMEM/F-12 containing 5% FBS, 40 nmol/ml dexamethasone, 20 ng/ml fibroblast growth factor (FGF)-4, 20 ng/ml hepatocyte growth factor (HGF), 10 ng/ml epidermal growth factor (EGF) (PeproTech), 1% insulin-transferrin-selenium-ethanolamine (ITS-X) and 1% 200 mM L-glutamine (Gibco; Thermo Fisher Scientific, Inc.) for 8, 12 and 15 days (21). Periodic acid-Schiff staining was conducted for hepatic glycogen, following the manufacturer's instructions.
For epithelial cell differentiation, cells were treated with DMEM/F-12 containing 10% FBS, 20 ng/ml EGF, 20 ng/ml bone morphogenetic protein (BMP)-7, 15 ng/ml insulin-like growth factor (IGF), 10 ng/ml LIF for 8, 10 and 12 days. Immunofluorescence analysis of polyclonal anti-CK18 and anti-CK19 antibodies (rabbit anti-chicken; dilution, 1:100; cat. nos. bs-1339R and bs-2190R, respectively; Beijing Biosynthesis Biotechnology Co. Ltd.) was performed (22).
The following pre-induction medium was used for islet differentiation: DMEM/F-12 containing 10 ng/ml bFGF, 10 ng/ml EGF, 2% B27 (Sigma-Aldrich) for 3 days, the terminal induction medium used was DMEM/F-12 containing 15 ng/ml HGF, 20 ng/ml activeA (Sigma-Aldrich), 1 mM β-mercaptoethanol (Sigma-Aldrich), 15 mM niacinamide (Gibco; Thermo Fisher Scientific, Inc.) and 2% B27. Dithizone staining (Peprotech) was performed to identify islet-like cells (23).
Following induction of the MMSCs, RNA extraction and RT-PCR identification were performed simultaneously, with specific marker genes used for different cell types. Gene-specific primer pairs are listed in Table I. GAPDH was used as the loading control.
Results
Morphological characterization of duck MMSCs
Primary cells began to adhere to plates at 16 h and proliferate 48 h later. After 3 passages, treatment with trypsin for a set period of time resulted in a purer population of MMSCs. Cells presented a fibroblast-like morphology, with long polygonal shapes, expanding rapidly until passage 15. Subsequent to this, cells presented signs of senescence (Fig. 1A–C).
Figure 1.
Morphology of primary and subcultured MMSCs and growth curve. (A) Following 16 h of primary culture, MMSCs adhered to plates. Hemocytes mixed with MMSCs were present. (B) Passage 3 MMSCs exhibited polygonal and long shuttle shapes, the majority of which presented protrusions, and gradually reached confluence, with strong cell proliferation ability. (C) Passage 8 MMSCs were fibroblast-like and homogeneous. Scale bars, 100 µm. (D) Growth curve for MMSCs. Cell proliferation processes are similar and typical of the ‘S’ shape. Data are presented as the mean ± standard deviation. n=3/group. MMSCs, metanephric mesenchymal stem cells; OD, optical density.
Growth curve
After 2 days of incubation, cellular growth follows an exponential pattern. Subsequent to day 6, growth begins to decline. Duck MMSCs follow the typical logistic growth pattern after 3 generations (Fig. 1D).
RT-PCR and immunofluorescence detection in the MMSCs
Cells from passages 5 and 10 exhibited no variation in the expression of the following markers: Fibronectin, Pax2, CD29, CD71, CD166, CD34 and CD45. All markers were expressed, with the exception of CD34 and CD45 (Fig. 2). As presented in Fig. 3, the MMSC-specific antigen markers fibronectin, CD29, CD71 and CD73 were positive, whilst the hematopoietic cell marker CD45 was negative.
Figure 2.
Reverse transcription-polymerase chain reaction analysis demonstrated that metanephric mesenchymal stem cells at passages 5 and 10 expressed fibronectin, Pax2, CD29, CD71 and CD166, but CD34 and CD45 were negative. GAPDH served as the internal control.
Figure 3.
Immunofluorescence identification of P5 generation cells at the same time points. (A) DAPI/without antibody, and (B) DAPI/with antibody. The results demonstrated that fibronectin, CD29, CD71 and CD73 were positive, whilst CD45 was negative. Scale bar, 50 µm.
Flow cytometry analysis in the MMSCs
The positive rate of specific surface markers was 97.19% for vimentin, 97.35% for CD44 and 97.95% for CD71 (Fig. 4).
Figure 4.
Flow cytometry analysis. The horizontal line on each histogram indicates the percentage of positive cells for each surface protein. FITC, fluorescein isothiocyanate.
Adipocyte differentiation of MMSCs
The control cell group gradually increased in size, with no significant change in cell morphology. Oil red O staining was negative (Fig. 5A). The growth of cells in the induced group was reduced on day 3, cells stretched to a flat shape, with a small number of oil droplets. By day 9, mast cells were more apparent, with shiny fat droplets (Fig. 5B). Cells were stained with 0.5% oil red O and fat droplets became more visible (Fig. 5C). On day 12, large fat droplets became evident, indicating that the cells had successfully differentiated into adipocytes. As expected, the induced group expressed PPAR-γ and LPL at days 6, 9 and 12; this was not evident in the control group (Fig. 5D).
Figure 5.
Morphology and gene expression of adipocytes following differentiation of MMSCs. (A) Negative control cells cultured in complete medium presented no changes in morphology and were negative for oil red O staining throughout the experimental period. (B) Following induction for 9 days, MMSCs became oblate and formed intracellular lipid droplets. At day 12, the mast cells possessed more apparent shiny fat droplets. (C) Lipid droplets, apparent at days 9 and 12, were stained with oil red O. Scale bars, 100 µm. (D) Expression of adipocyte-specific genes. LPL and PPAR-γ were detected in the induced group following induction for 6, 9 or 12 days, but not expressed in the control group. GAPDH served as the internal control. MMSCs, metanephric mesenchymal stem cells; LPL, lipoprotein lipase; PPAR-γ, peroxisome proliferator-activated receptor-γ.
Hepatocyte differentiation of MMSCs
The control group exhibited no changes in morphology, and staining results were negative for the duration of the experiment (Fig 6A). In the induced group, cells with polygonal shape increased in numbers on day 8 of the induction. On day 15 of the induction, the distinctive cubic hepatocyte shape became apparent (Fig. 6B and C). Successful periodic acid-Schiff staining confirmed the glycogen synthesis and storage functions of the induced cells (Fig. 6D). Gene expression of AFP and ALB confirmed the differentiation of MMSCs into hepatocytes in the induced group, whilst control cells did not express any hepatocyte-specific genes (Fig. 6E).
Figure 6.
Morphology of hepatocyte differentiation of metanephric mesenchymal stem cells and specific gene expression following induction for 15 days. (A) In the negative control group, cells presented no clear change in form and were negative for periodic acid-Schiff staining. Scale bar, 100 µm. (B and C) In the induced group, the number of round cells significantly increased, presenting a polygonal shape. (B) Scale bar, 100 µm; (C) scale bar, 50 µm. (D) Periodic acid-Schiff staining was successful. Scale bar, 100 µm. (E) Hepatocyte-specific genes ALB and AFP were expressed in the induced group following induction for 8, 12 or 15 days. These genes were not expressed in the control group. GAPDH served as the internal control. With the increase of induction days, gene expression strengthened gradually.
Epithelial cell differentiation of MMSCs
On day 10, the epithelioid cells were plate-shaped and exhibited single-layer adherent growth, whilst the control cells did not differentiate (Fig. 7A and B). Immunofluorescence analysis demonstrated that the induced cells were positive for CK18 and CK19 (Fig. 7C–F). Expression of E-cadherin and CK19 was also detected, by PCR analysis (Fig. 7G).
Figure 7.
Morphology and gene expression of epithelial cells following differentiation of metanephric mesenchymal stem cells. (A) The control group presented no clear change in form. Scale bar, 100 µm. (B) In the induced group, following 10 days, epithelioid cells were plates like pebbles or paving stones, with single-layer adherent growth. Normal clear cell boundary and refraction, stereoscopic, closely linked cells. Scale bar, 100 µm. Immunofluorescence analysis: (C) DAPI/without antibody; (D) CK18+/DAPI; (E) DAPI/without antibody; (F) CK19+/DAPI. Scale bars, 50 µm. (G) Epithelial cell-specific genes CK19 and E-cadherin were expressed in the induced group following 8-, 10- and 12-day induction, but the control group was negative.
Islet cell differentiation of MMSCs
Following pre-induction, cells expanded rapidly. The addition of the terminal medium limited cell growth and caused the formation of clusters. Control cells did not differentiate morphologically (Fig. 8A). Cell clusters were observed, and stained red by dithizone (Fig. 8B and C). PDX-1 and insulin were expressed in differentiated cells but not in the control group (Fig. 8D).
Figure 8.
Morphology and specific gene expression of islet cells following differentiation of metanephric mesenchymal stem cells. (A) The control group presented no significant change in form. (B) Following induction for 10 days, there were several islet-like cell clusters in the induced cell plate. (C) Cell clusters were dyed scarlet by dithizone staining. Scale bars, 100 µm. (D) Islet cell-specific genes PDX-1 and insulin were positive in the induced group, but not in the control. GAPDH served as the internal control.
Discussion
In the current study, MMSCs were isolated from 20-day-old Beijing duck metanephros and assessed for their self-renewal potential, biological characteristics and differentiation abilities. The duck embryo is a classic model of vertebrate developmental biology, which has been used extensively (24). Enzymatic digestion was used as a stable method to harvest MMSCs from metanephric tissues. Cell lines exhibited typical logistic growth, with an S-shaped growth curve, in accordance with previously observed in vitro cell growth patterns (25). Due to mechanical and chemical damage caused by external conditions in subculture, cells require adaptation and recovery periods. Increasing cell passages, and external environmental effects cause cells to gradually age (26).
At present, specific surface markers of MMSCs are unknown, and MMSCs are generally identified based on the expression of certain markers that have been previously associated with MSCs. The present study used immunofluorescence, RT-PCR and flow cytometry to detect whether MMSCs possessed the surface characteristics of MSCs. MMSCs were long and fusiform, with a small number of polygonal cells. Furthermore, they exhibited growth trends similar to MSCs (27). MMSCs in the present study were indicated to have morphological characteristics comparable to MSCs. CD29 (also known as integrin β1) is a β-subunit protein and a member of the integrin family. CD29 can form a heterodimer with surface and extracellular proteins, such as CD49 and CD51, in MSCs in order to mediate the cell-cell and cell-matrix interactions. In the present study, CD29, CD71 and CD44 levels were detected as the surface markers of MMSCs using immunofluorescence and RT-PCR, which were shown to have a sensitive expression in different passages. Therefore, CD29, CD71 and CD44 may be considered as specific makers of MMSCs.
Depending on induction conditions, MMSCs are able to differentiate into various cell types. MMSCs originate from the mesoderm and are pluripotent, meaning they can differentiate into ectoderm, mesoderm and endoderm cells (28). In the present study, MMSCs were induced into adipocytes, hepatocytes, epithelial cells and islet cells. The induced cells illustrated typical staining and expressed specific marker genes.
Oil red O staining is a method used for the identification of lipid droplets in cells. IBMX increases levels of cAMP to regulate the expression of PPAR-γ. In the present study, PPAR-γ and LPL were expressed in the early stages of adipogenic differentiation. This suggests that MMSCs may be induced to differentiate into adipocytes under appropriate conditions and induction times.
Liver cells (hepatocytes) can produce and store glycogen, and the induced cells in the current study were identified to contain glycogen by periodic acid-Schiff staining. This indicated that these cells have a glycogen synthesis and storage function.
HGF induces mitosis and prevents apoptosis in hepatocytes, boosting glycogen formation, while FGF-4 is an important factor for hepatocyte growth and development. The present study identified positive expression of AFP and ALB, which may indicate that hepatocyte differentiation is associated with gene expression. AFP expression was higher in the early stages of differentiation, indicating that AFP may serve a regulatory role in liver-like cells during early induction processes. This raises the question whether hepatocyte differentiation is associated with gene expression to a certain degree.
EGF is able to promote mitosis and proliferation in a variety of cells, stimulating the division of keratinocytes in vitro and in vivo and promoting epithelial cell regeneration. IGF, another common growth factor, possesses various physiological functions, including the regulation of body growth and the promotion of mitosis in cell cultures. Collectively, IGF, EGF and BMP-7 can function to induce the differentiation of MMSCs to epithelial cells, in addition to inhibiting fibroblast growth and promoting epithelial cell growth and proliferation (29). Cytokeratins maintain the structural integrity of keratinized epithelium, stratified squamous epithelium, stratified epithelium, hyperplastic cutinized epithelium and simple epithelium (30).
In the present study, pancreatic islet cells were induced by adding HGF, active A and niacinamide. Active A activates β-cells in the pancreatic islets, while niacinamide aids the converging and expanding of islet cells (31). Dithizone staining revealed scarlet stained cell clusters, indicating that the induced cell cytoplasm possessed zinc ions, corresponding to islet cell characteristics (32). The induced cells also expressed PDX-1 and insulin. Further research should focus on improving β-cell induction and promoting insulin secretion, which may benefit patients with diabetes.
In conclusion, the Beijing duck embryo MMSC cultivation system was established in the present study. MMSCs were found to express MSC marker genes, which was determined using RT-PCR, immunofluorescence and flow cytometry. In addition, MMSCs were found to have a self-proliferation and multidirectional differentiation potential. MMSCs may be applicable to kidney transplantation for kidney disease treatment in the future, since they are able to repair the damage of kidney tissue and help restore the normal kidney function.
Acknowledgements
The current research was supported by the Agricultural Science and Technology Innovation Program (ASTIP; cxgc-ias-01), the National Natural Science Foundation of China (grant no. 31472099), the National Infrastructure of Animal Germplasm Resources (2014).
References
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