Abstract
If native or heparinized blood is passed slowly through a column of glass beads at room temperature, the number of platelets removed from the initial drop emerging from the column is less than that removed from the final drop. At 4°C. this difference disappears. If the blood is passed rapidly through such columns at room temperature fewer platelets are removed, but the initial-final difference persists. Von Willebrand's platelets are removed normally at slow speeds; at fast speeds abnormally few platelets are removed. Platelets emerging from all such columns are in aggregates.
On adding glass beads to normal heparinized plasma, the platelets at once become more rounded and after about 50 seconds' delay they aggregate: the delay and rate of aggregation can be quantitated. Aggregation occurs best at 20 to 30°C. and is not inhibited by the addition of some enzyme inhibitors. In von Willebrand's disease all these glass-induced aggregation phenomena occur normally and aggregation in response to adenosine diphosphate (ADP), serotonin creatinine sulphate (5-HT), adrenaline, collagen, and glass is also normal.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BORN G. V. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. 1962 Jun 9;194:927–929. doi: 10.1038/194927b0. [DOI] [PubMed] [Google Scholar]
- Bull B. S., Zucker M. B. Changes in platelet volume produced by temperature, metabolic inhibitors, and aggregating agents. Proc Soc Exp Biol Med. 1965 Nov;120(2):296–301. doi: 10.3181/00379727-120-30516. [DOI] [PubMed] [Google Scholar]
- FEISSLY R., LUDIN H. Microscopie par contrastes de phases; applications à l'hématologie. Rev Hematol. 1949;4(3):481–501. [PubMed] [Google Scholar]
- O'BRIEN J. R. The adhesiveness of native platelets and its prevention. J Clin Pathol. 1961 Mar;14:140–149. doi: 10.1136/jcp.14.2.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALZMAN E. W. MEASUREMENT OF PLATELET ADHESIVENESS. A SIMPLE IN VITRO TECHNIQUE DEMONSTRATING AN ABNORMALITY IN VON WILLEBRAND'S DISEASE. J Lab Clin Med. 1963 Nov;62:724–735. [PubMed] [Google Scholar]
- STRAUSS H. S., BLOOM G. E. VON WILLEBRAND'S DISEASE: USE OF A PLATELET-ADHESIVENESS TEST IN DIAGNOSIS AND FAMILY INVESTIGATION. N Engl J Med. 1965 Jul 22;273:171–181. doi: 10.1056/NEJM196507222730401. [DOI] [PubMed] [Google Scholar]
- ZUCKER M. B. In vitro abnormality of the blood in von Willebrand's disease correctable by normal plasma. Nature. 1963 Feb 9;197:601–602. doi: 10.1038/197601a0. [DOI] [PubMed] [Google Scholar]