Abstract
A method is described for the determination of serum thyroxine using ion-exchange resin sponges. The principle is based on the use of protein-binding sites and part of the technique is similar to the Triomet resin uptake test. An isotope extraction recovery procedure is incorporated in the method. Studies of factors influencing the analysis are reported and the results of tests of accuracy, precision, and specificity are presented. Serum thyroxine iodine values are compared with protein-bound iodine estimations. The values found in health and disease are similar to the results of previous workers.
The specificity of the analysis represents a considerable advantage over protein-bound iodine determinations since no interference is caused by iodides, iodine-containing contrast media, mercurial diuretics, mono-iodotyrosine, di-iodotyrosine, di-iodothyronine, and also tri-iodothyronine in physiological amounts. Therapy with diphenylhydantoin and tri-iodothyronine will tend to depress protein-bound iodine levels and thyroxine values are similarly influenced.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- EKINS R. P. The estimation of thyroxine in human plasma by an electrophoretic technique. Clin Chim Acta. 1960 Jul;5:453–459. doi: 10.1016/0009-8981(60)90051-6. [DOI] [PubMed] [Google Scholar]
- FARRELL L. P., RICHMOND M. H. A rapid method for the estimation of serum protein-bound iodine. Clin Chim Acta. 1961 Sep;6:620–623. doi: 10.1016/0009-8981(61)90104-8. [DOI] [PubMed] [Google Scholar]
- MURPHY B. E., PATTEE C. J. DETERMINATION OF THYROXINE UTILIZING THE PROPERTY OF PROTEIN-BINDING. J Clin Endocrinol Metab. 1964 Feb;24:187–196. doi: 10.1210/jcem-24-2-187. [DOI] [PubMed] [Google Scholar]
- Nakajima H., Kuramochi M., Horiguchi T., Kubo S. A new and simple method for the determination of thyroxine in serum. J Clin Endocrinol Metab. 1966 Jan;26(1):99–103. doi: 10.1210/jcem-26-1-99. [DOI] [PubMed] [Google Scholar]
- OPPENHEIMER J. H., FISHER L. V., NELSON K. M., JAILER J. W. Depression of the serum protein-bound iodine level by diphenylhydantion. J Clin Endocrinol Metab. 1961 Mar;21:252–262. doi: 10.1210/jcem-21-3-252. [DOI] [PubMed] [Google Scholar]