Abstract
Whole blood was heated for twenty minutes at 40°C., 45°C., 50°C., and 55°C. Changes in the osmotic resistance of the heat-exposed cells were then determined. The values obtained were plotted as lysis increments. This treatment revealed groups of cells of varying heat sensitivity. After heating to 50°C., cell groups were therefore prepared by partitioning cells between layers of a mixture of methyl- and butyl-phthalates of known densities. Three cell groups enriched in either oldest cells, cell fragments, or cell fragments and youngest cells were obtained. These groups subjected to serialosmotic lysis tests revealed that the densest, i.e., oldest fraction, had least thermal resistance. Fractionation and osmotic resistance studies provided clear evidence of some thermal damage to erythrocytes of median age, i.e., cells which would normally be expected to remain in the circulation for another two months. Some of the possible implications are discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CONNERTY H. V., BRIGGS A. R. New method for the determination of whole-blood iron and hemoglobin. Clin Chem. 1962 Apr;8:151–157. [PubMed] [Google Scholar]
- CRUICKSHANK C. N., HERSHEY F. B. The effect of heat on the metabolism of guinea pig's ear skin. Ann Surg. 1960 Mar;151:419–430. doi: 10.1097/00000658-196003000-00017. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DANON D., MARIKOVSKY V. DETERMINATION OF DENSITY DISTRIBUTION OF RED CELL POPULATION. J Lab Clin Med. 1964 Oct;64:668–674. [PubMed] [Google Scholar]
- DAVIES J. W., TOPLEY E. The disappearance of red cells in patients with burns. Clin Sci. 1956 Feb;15(1):135–148. [PubMed] [Google Scholar]
- DRABKIN D. L. THE MOLECULAR WEIGHT OF HAEMOGLOBIN, ITS IRON AND NITROGEN CONTENT AND OPTICAL PROPERTIES--THEIR RELEVANCE IN THE PROBLEM OF A REFERENCE STANDARD FOR HAEMOGLOBIN MEASUREMENT. Bibl Haematol. 1965;21:33–42. doi: 10.1159/000428151. [DOI] [PubMed] [Google Scholar]
- FISCHL J. Routine microestimation of iron in hemoglobin. Clin Chim Acta. 1959 Sep;4:686–690. doi: 10.1016/0009-8981(59)90010-5. [DOI] [PubMed] [Google Scholar]
- Isaacs R., Brock B., Minot G. R. THE RESISTANCE OF IMMATURE ERYTHROCYTES TO HEAT. J Clin Invest. 1925 Jun;1(5):425–433. doi: 10.1172/JCI100022. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KIMBER R. J., LANDER H. THE EFFECT OF HEAT ON HUMAN RED CELL MORPHOLOGY, FRAGILITY, AND SUBSEQUENT SURVIVAL IN VIVO. J Lab Clin Med. 1964 Dec;64:922–933. [PubMed] [Google Scholar]
- MUIR I. F. Red-cell destruction in burns, with particular reference to the shock period. Br J Plast Surg. 1961 Dec;14:273–302. doi: 10.1016/s0007-1226(61)80051-9. [DOI] [PubMed] [Google Scholar]
- Moore F. D., Peacock W. C., Blakely E., Cope O. The Anemia of Thermal Burns. Ann Surg. 1946 Nov;124(5):811–839. [PMC free article] [PubMed] [Google Scholar]
- Parpart A. K., Lorenz P. B., Parpart E. R., Gregg J. R., Chase A. M. THE OSMOTIC RESISTANCE (FRAGILITY) OF HUMAN RED CELLS. J Clin Invest. 1947 Jul;26(4):636–640. doi: 10.1172/JCI101847. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SPEAR P. W., SASS M. D. SOME CURRENT CONCEPTS OF RED CELL METABOLISM. I. METABOLIC PROCESSES IN THE RED CELL. Metabolism. 1964 Oct;13:911–928. doi: 10.1016/0026-0495(64)90081-2. [DOI] [PubMed] [Google Scholar]