Abstract
Thirteen isolations of Toxoplasma gondii were made from 110 specimens of various human tissues and tissue fluids. Twelve of these were from lymph nodes and one from a specimen of cerebrospinal fluid; all isolations were from cases with dye test titres of 1/512 or more. A description of the deep freeze method used to preserve the T. gondii strains isolated is included. It is hoped that attempts at isolation of this organism will be made more frequently in future.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BEVERLEY J. K., BEATTIE C. P. Glandular toxoplasmosis; a survey of 30 cases. Lancet. 1958 Aug 23;2(7043):379–384. doi: 10.1016/s0140-6736(58)90109-0. [DOI] [PubMed] [Google Scholar]
- CHANDLER A. H., WEINMAN D. Prolonged storage of toxoplasma at -70 C.; a preliminary report. Am J Clin Pathol. 1956 Mar;26(3):323–326. doi: 10.1093/ajcp/26.3_ts.323. [DOI] [PubMed] [Google Scholar]
- DOUGHERTY R. M. Use of dimethyl sulphoxide for preservation of tissue culture cells by freezing. Nature. 1962 Feb 10;193:550–552. doi: 10.1038/193550a0. [DOI] [PubMed] [Google Scholar]
- EYLES D. E., COLEMAN N., CAVANAUGH D. J. Preservation of Toxoplasma gondii by freezing. J Parasitol. 1956 Aug;42(4 SECTION):408–413. [PubMed] [Google Scholar]
- LANGER H. Repeated congenital infection with toxoplasma gondii. Obstet Gynecol. 1963 Mar;21:318–329. [PubMed] [Google Scholar]
- LELONG M., BERNARD J., DESMONTS G., COUVREUR J. [Acquired toxoplasmosis. (Study of 227 cases)]. Arch Fr Pediatr. 1960;17:281–331. [PubMed] [Google Scholar]
- PORTERFIELD J. S., ASHWOOD-SMITH M. J. Preservation of cells in tissue culture by glycerol and dimethyl sulphoxide. Nature. 1962 Feb 10;193:548–550. doi: 10.1038/193548a0. [DOI] [PubMed] [Google Scholar]
- SIIM J. C. Pathogenetic aspects of human acquired toxoplasmosis. Surv Ophthalmol. 1961 Dec;6:781–798. [PubMed] [Google Scholar]
- WALKER P. J., ASHWOOD-SMITH M. J. Dimethyl sulphoxide, an alternative to glycerol, for the low-temperature preservation of trypanosomes. Ann Trop Med Parasitol. 1961 Apr;55:93–96. doi: 10.1080/00034983.1961.11686023. [DOI] [PubMed] [Google Scholar]