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. 1993 Sep 15;90(18):8332–8336. doi: 10.1073/pnas.90.18.8332

Polarization of yeast cells in spatial gradients of alpha mating factor.

J E Segall 1
PMCID: PMC47350  PMID: 8397402

Abstract

The process of cell fusion during mating of the yeast Saccharomyces cerevisiae is mediated by factors secreted by the mating partners. Spatial gradients of one of these mating factors, alpha-factor, polarized the growth of projections by MATa cells. The site of previous budding did not affect the direction of polarization, and subsequent budding was also polarized if mating factor was removed. Orientation occurred in the presence of nocodazole, suggesting that microtubules were not critical. At extremely low concentrations of alpha-factor, sst2-mutants (which in genetic studies do not discriminate between partners producing different amounts of alpha-factor) were able to polarize their projections. The sensitivity of this spatial sensing mechanism in wild-type cells is such that differences in receptor occupancy estimated to be about 1% are sufficient for orientation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe K., Kusaka I., Fukui S. Morphological change in the early stages of the mating process of Rhodosporidium toruloides. J Bacteriol. 1975 May;122(2):710–718. doi: 10.1128/jb.122.2.710-718.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chan R. K., Otte C. A. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Mol Cell Biol. 1982 Jan;2(1):11–20. doi: 10.1128/mcb.2.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chang F., Herskowitz I. Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2. Cell. 1990 Nov 30;63(5):999–1011. doi: 10.1016/0092-8674(90)90503-7. [DOI] [PubMed] [Google Scholar]
  4. Cross F., Hartwell L. H., Jackson C., Konopka J. B. Conjugation in Saccharomyces cerevisiae. Annu Rev Cell Biol. 1988;4:429–457. doi: 10.1146/annurev.cb.04.110188.002241. [DOI] [PubMed] [Google Scholar]
  5. Dutcher S. K., Hartwell L. H. Genes that act before conjugation to prepare the Saccharomyces cerevisiae nucleus for caryogamy. Cell. 1983 May;33(1):203–210. doi: 10.1016/0092-8674(83)90349-5. [DOI] [PubMed] [Google Scholar]
  6. Herman A. I. Sex-specific growth responses in yeasts. Antonie Van Leeuwenhoek. 1971;37(4):379–384. doi: 10.1007/BF02218508. [DOI] [PubMed] [Google Scholar]
  7. Jackson C. L., Hartwell L. H. Courtship in S. cerevisiae: both cell types choose mating partners by responding to the strongest pheromone signal. Cell. 1990 Nov 30;63(5):1039–1051. doi: 10.1016/0092-8674(90)90507-b. [DOI] [PubMed] [Google Scholar]
  8. Jackson C. L., Hartwell L. H. Courtship in Saccharomyces cerevisiae: an early cell-cell interaction during mating. Mol Cell Biol. 1990 May;10(5):2202–2213. doi: 10.1128/mcb.10.5.2202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jenness D. D., Goldman B. S., Hartwell L. H. Saccharomyces cerevisiae mutants unresponsive to alpha-factor pheromone: alpha-factor binding and extragenic suppression. Mol Cell Biol. 1987 Apr;7(4):1311–1319. doi: 10.1128/mcb.7.4.1311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Konopka J. B., Jenness D. D., Hartwell L. H. The C-terminus of the S. cerevisiae alpha-pheromone receptor mediates an adaptive response to pheromone. Cell. 1988 Aug 26;54(5):609–620. doi: 10.1016/s0092-8674(88)80005-9. [DOI] [PubMed] [Google Scholar]
  11. Kurjan J. Pheromone response in yeast. Annu Rev Biochem. 1992;61:1097–1129. doi: 10.1146/annurev.bi.61.070192.005313. [DOI] [PubMed] [Google Scholar]
  12. MORTIMER R. K. Evidence of two types of x-ray-induced lethal damage in Saccharomyces cerevisiae. Radiat Res. 1955 Jun;2(4):361–368. [PubMed] [Google Scholar]
  13. Marsh L., Neiman A. M., Herskowitz I. Signal transduction during pheromone response in yeast. Annu Rev Cell Biol. 1991;7:699–728. doi: 10.1146/annurev.cb.07.110191.003411. [DOI] [PubMed] [Google Scholar]
  14. Müller I. Parental age and the life-span of zygotes of Saccharomyces cerevisiae. Antonie Van Leeuwenhoek. 1985;51(1):1–10. doi: 10.1007/BF00444223. [DOI] [PubMed] [Google Scholar]
  15. Trueheart J., Boeke J. D., Fink G. R. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein. Mol Cell Biol. 1987 Jul;7(7):2316–2328. doi: 10.1128/mcb.7.7.2316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Watzele M., Klis F., Tanner W. Purification and characterization of the inducible a agglutinin of Saccharomyces cerevisiae. EMBO J. 1988 May;7(5):1483–1488. doi: 10.1002/j.1460-2075.1988.tb02966.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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