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. 1967 Sep;20(5):693–698. doi: 10.1136/jcp.20.5.693

A comparison of two methods for the immunoassay of intrinsic factor

K D Bardhan 1, A G Wangel 1, Sheila T Callender 1
PMCID: PMC473553  PMID: 5602977

Abstract

The intrinsic factor content of 263 samples of gastric juice was determined by immunoassay using charcoal absorption and by immunoelectrophoresis on acrylamide gel. There was good correlation between the results of the two techniques and, with a few exceptions, both made it possible to predict which patients would have malabsorption of radioactive vitamin B12.

Of the two methods, immunoassay using charcoal absorption generally gave the higher readings. Further study showed that this could not be attributed to the recognized difference in the principles of the two methods but that it was more likely to be due to the dissimilarity of the experimental conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARDEMAN S., CHANARIN I. A METHOD FOR THE ASSAY OF HUMAN GASTRIC INTRINSIC FACTOR AND FOR THE DETECTION AND TITRATION OF ANTIBODIES AGAINST INTRINSIC FACTOR. Lancet. 1963 Dec 28;2(7322):1350–1354. doi: 10.1016/s0140-6736(63)90736-0. [DOI] [PubMed] [Google Scholar]
  2. Ardeman S., Chanarin I., Jacobs A., Griffiths L. Family study in Addisonian pernicious anemia. Blood. 1966 May;27(5):599–610. [PubMed] [Google Scholar]
  3. Ardeman S. Studies on gastric intrinsic factor. Proc R Soc Med. 1965 Nov;58(11 Pt 1):916–917. doi: 10.1177/003591576505811P129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bardhan K. D., Callender S. T., Spray G. H. Simplified technique of immuno-electrophoretic assay of human intrinsic factor on acrylamide gel. Gut. 1966 Oct;7(5):566–568. doi: 10.1136/gut.7.5.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Callender S. T., Witts L. J., Warner G. T., Oliver R. The use of a simple whole-body counter for haematological investigations. Br J Haematol. 1966 May;12(3):276–282. doi: 10.1111/j.1365-2141.1966.tb05634.x. [DOI] [PubMed] [Google Scholar]
  6. GORDIN R. Vitamin B12 absorption in corticosteroid-treated pernicious anaemia. Acta Med Scand. 1959 Jun 6;164(2):159–165. doi: 10.1111/j.0954-6820.1959.tb00175.x. [DOI] [PubMed] [Google Scholar]
  7. JEFFRIES G. H., SLEISENGER M. H. The immunologic identification and quantitation of human intrinsic factor in gastric secretions. J Clin Invest. 1963 Apr;42:442–449. doi: 10.1172/JCI104732. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. OSTERGAARD KRISTENSEN H. P., FRIIS T. Effect of prednisone on B12 absorption in pernicious anaemia. Acta Med Scand. 1960 Mar 30;166:249–254. doi: 10.1111/j.0954-6820.1960.tb17376.x. [DOI] [PubMed] [Google Scholar]
  9. Rodbro P., Christiansen P. M. Intrinsic-factor secretion in stomach diseases. Lancet. 1965 Dec 11;2(7424):1200–1203. doi: 10.1016/s0140-6736(65)90631-8. [DOI] [PubMed] [Google Scholar]
  10. Warner G. T., Oliver R. A whole-body counter for clinical measurements utilizing the 'shadow shield' technique. Phys Med Biol. 1966 Jan;11(1):83–94. doi: 10.1088/0031-9155/11/1/307. [DOI] [PubMed] [Google Scholar]

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