Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1967 Nov;20(6):905–909. doi: 10.1136/jcp.20.6.905

Semi-automated method for the determination of free fatty acids in plasma

Joyce D Baird 1,2,3, M W Black 1,2,3, D E Faulkner 1,2,3
PMCID: PMC473637  PMID: 5614076

Abstract

A semi-automated method for the determination of free fatty acids in plasma based on Mosinger's manual method (Mosinger, 1965) is described and the results of a comparison with Dole's titrimetric method (Dole, 1956) are presented. There is almost perfect correlation between the two methods, and the automated technique is simple, reliable, and significantly more accurate and precise than the titrimetric method.

Full text

PDF
905

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANTONIS A. SEMIAUTOMATED METHOD FOR THE COLORIMETRIC DETERMINATION OF PLASMA FREE FATTY ACIDS. J Lipid Res. 1965 Apr;6:307–312. [PubMed] [Google Scholar]
  2. DOLE V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest. 1956 Feb;35(2):150–154. doi: 10.1172/JCI103259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DOLE V. P., MEINERTZ H. Microdetermination of long-chain fatty acids in plasma and tissues. J Biol Chem. 1960 Sep;235:2595–2599. [PubMed] [Google Scholar]
  4. Duncombe W. G. The colorimetric micro-determination of long-chain fatty acids. Biochem J. 1963 Jul;88(1):7–10. doi: 10.1042/bj0880007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GORDON R. S., Jr Unesterified fatty acid in human blood plasma. II. The transport function of unesterified fatty acid. J Clin Invest. 1957 Jun;36(6 Pt 1):810–815. doi: 10.1172/JCI103486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ITAYA K., UI M. COLORIMETRIC DETERMINATION OF FREE FATTY ACIDS IN BIOLOGICAL FLUIDS. J Lipid Res. 1965 Jan;6:16–20. [PubMed] [Google Scholar]
  7. MOSINGER F. PHOTOMETRIC ADAPTATION OF DOLE'S MICRODETERMINATION OF FREE FATTY ACIDS. J Lipid Res. 1965 Jan;6:157–159. [PubMed] [Google Scholar]
  8. TAYLOR I. E., MARSH M. M. The solution of problems involving special handling of samples and reagents in an automatic system. Ann N Y Acad Sci. 1960 Jul 22;87:775–781. doi: 10.1111/j.1749-6632.1960.tb23234.x. [DOI] [PubMed] [Google Scholar]
  9. TROUT D. L., ESTES E. H., Jr, FRIEDBERG S. J. Titration of free fatty acids of plasma: a study of current methods and a new modification. J Lipid Res. 1960 Apr;1:199–202. [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES