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Biophysics and Physicobiology logoLink to Biophysics and Physicobiology
. 2015 Dec 11;12:121–129. doi: 10.2142/biophysico.12.0_121

Microbial rhodopsins: wide distribution, rich diversity and great potential

Marie Kurihara 1, Yuki Sudo 1,2,
PMCID: PMC4736836  PMID: 27493861

Abstract

One of the major topics in biophysics and physicobiology is to understand and utilize biological functions using various advanced techniques. Taking advantage of the photoreactivity of the seven-transmembrane rhodopsin protein family has been actively investigated by a variety of methods. Rhodopsins serve as models for membrane-embedded proteins, for photoactive proteins and as a fundamental tool for optogenetics, a new technology to control biological activity with light. In this review, we summarize progress of microbial rhodopsin research from the viewpoint of distribution, diversity and potential.

Keywords: retinal, rhodopsin, π-conjugation, visible light, vitamin-A

Rhodopsins

In the early morning, we usually wake up with bright sunlight arising from the horizon, take breakfast and go to the office or school. On the way to the office or school, we are able to see colorful plants and animals. We then make a living for several hours under illumination with fluorescent and/or light emitting diode (LED) bulbs or with sunlight. On the way back home, we can be fascinated by the incredible view of a setting sun. At night, beautiful fireworks in the sky give us hope for tomorrow. Thus, light is one of the most important stimuli for organisms.

In organisms, including human beings, biological protein molecules are responsible for the light reception. However, in general, proteins are transparent and are thus not responsive in the visible light region. Therefore, photoreceptive proteins have evolved that can bind a chromophore molecule, which can absorb visible wavelengths of light. One of the largest groups of photoreceptive proteins is the rhodopsin protein family (also called retinal protein), where vitamin-A aldehyde retinal is employed as a chromophore [1]. The retinal chromophore forms the all-trans configuration in organic solvents as the most favorable conformer and it absorbs blue light (~360 nm) (Fig. 1a) [1]. The retinal is incorporated into the protein moiety (called an “opsin”) that consists of seven-transmembrane α-helices by the formation of a protonated Schiff base linkage to a specific conserved lysine residue in the opsin (Fig. 1b). The interaction between retinal and opsin induces spectral red-shifts in a wide range of the visible region (400~600 nm) [2] (Fig. 1c). The opsin-induced spectral shift is called an “opsin-shift”. The visible light absorption of rhodopsins triggers the trans-cis or cis-trans isomerization of the retinal chromophore [1]. The isomerization process appears within a few hundreds of a femtosecond time frame. The stored energy of the excited retinal chromophore induces sequential conformational changes of the protein moiety required for its biological functions, such as visual responses, ion transportation and photosensing [1,2].

Figure 1.

Figure 1

Introduction of the photoactive rhodopsin molecule. (a) Chemical structure of the retinal chromophore with numbering of the carbons. (b) Crystal structure of a typical type-1 microbial rhodopsin (bacteriorhodopsin, BR: PDB code, 1C3W) [71]. The retinal chromophore (yellow) that is covalently attached to the opsin via a specific lysine residue is surrounded by seven-transmembrane α-helices (A, B, C, D, E, F and G). (c) Schematic of the opsin-induced spectral shift of the retinal chromophore, named the “opsin-shift”. The absorption spectra from the retinal chromophore colored yellow in solution are greatly affected by interaction with a variety of opsins.

It is well-known that there are two types of rhodopsins in nature [3]. One is type-1 rhodopsins, which are widespread in the microbial world [4,5], while the other is type-2 rhodopsins, which are widely distributed in animals [6,7] and are categorized as members of the G-protein-coupled receptor (GPCR) family. As mentioned above, all-trans retinal is the most stable conformer, and therefore many type-1 rhodopsins have the all-trans configuration in the dark (Fig. 1b) [13]. On the other hand, many type-2 rhodopsins have the 11-cis configuration due to the specific interaction with the opsins [1,6,7]. In addition to the chromophore difference, sequence homology between type-1 and type-2 rhodopsins is low, though both possess similar chromophore (retinal) and protein (seven-transmembrane helices) structures [8].

In this review, we would like to focus on type-1 microbial rhodopsins. Please see extensive reviews for type-2 rhodopsins [1,6,7,9,10].

Classical type-1 rhodopsins from the archaeon Halobacterium salinarum

In 1971, the first type-1 rhodopsin, named bacteriorhodopsin (BR), was found in the halophilic archaeon Halobacterium salinarum (formerly halobium) [11]. BR works as a light-driven outward electrogenic proton pump in the cell membrane, and one H+ moves from the intracellular to the extracellular side during a cyclic photoreaction called a “photocycle” [12]. The proton gradient produced is utilized by adenosine triphosphate (ATP) synthase. In other words, organisms having light-driven pumps can produce ATP under light illumination (Fig. 2). It is well-known that ATP is a multifunctional nucleotide used in organisms as a coenzyme, and is often called the molecular unit of currency of intracellular energy transfer.

Figure 2.

Figure 2

The classical four microbial rhodopsins from the archaeon Halobacterium salinarum. The membrane of H. salinarum contains four rhodopsins, bacteriorhodopsin (BR), halorhodopsin (HR), sensory rhodopsin-I (SRI) and sensory rhodopsin-II (SRII, also called phoborhodopsin, pR). BR and HR work as a light-driven proton pump and a halide ion pump, respectively, while SRI and SRII work as photo-sensors, and form signaling complexes with their cognate transducer proteins, HtrI and HtrII, respectively, in the membrane. Light signals are transmitted from the SRI-HtrI and SRII-HtrII complexes to a cytoplasmic two-component signal transduction cascade that consists of the adaptor protein CheW, the kinase CheA and the response regulator CheY, which regulates the rotational direction of the flagellar motor, resulting in attractant or repellent phototaxis responses. Adaptation is also essential for the detection of temporal changes of stimuli and high sensitivity to stimuli over a wide dynamic range. Covalent modifications of Htrs (i.e., methylation and demethylation), which are involved in adaptation, are illustrated as “CH3”. “P” indicates the phosphate functional group. Using those signaling systems, cells move toward longer wavelengths of light (λ>520 nm) where BR and HR work to produce ATP, while they avoid shorter wavelengths of light (λ<520 nm), which contain harmful near-UV.

From the same organism, three other rhodopsins, halorhodopsin (HR), sensory rhodopsin-I (SRI) and sensory rhodopsin-II (SRII), were discovered in 1977 [13], 1982 [14] and 1985 [15], respectively (Fig. 2). HR works as a light-driven inward electrogenic chloride pump and produces ATP as does BR [16,17]. However the physiological role of HR is not fully understood. HR-induced ATP synthesis is sustained for only few minutes. Therefore, besides the ATP production, the Cl transport has been suggested to contribute in maintaining the osmotic balance during the cell growth [16]. SRI and SRII initiate positive or negative phototaxis responses, respectively [18]. When a cell moves toward a certain wavelength of light, this behavior is designated as positive phototaxis, while behavior in which a cell avoids a certain wavelength of light is designated as negative phototaxis. SRI and SRII form a 2 : 2 signaling complex in the membrane with their cognate transducer proteins HtrI and HtrII, respectively (Fig. 2) [1921]. Light absorption by SRI and SRII triggers the trans-cis photo-isomerization of retinal chromophores, leading to the cognate photocycle. During the photocycle, light signals are transmitted from the SRI-HtrI and SRII-HtrII complexes to the cytoplasmic two-component signal transduction cascade, which consists of the kinases CheA and CheY with an adaptor protein CheW (Fig. 2) [18]. Finally, the rotational direction of the flagellar motor is regulated by the phosphorylation state of CheY (Fig. 2) [18]. SRII absorbs a shorter wavelength of light (~500 nm), whereas BR, HR and SRI absorb a longer one (~580 nm) [18,22,23]. As a result, the archaeon Halobacterium salinarum is attracted to light with wavelengths longer than 520 nm and avoids light with wavelengths shorter than 520 nm (Fig. 2) [18]. Light of >520 nm can activate BR and HR to produce ATP, and cells avoid shorter wavelengths of light which contain harmful near-UV [18]. The close relationship between these four archaeal rhodopsins (BR, HR, SRI and SRII) has been experimentally demonstrated by functional conversion with replacement of some amino acid residues (i.e., conversion from BR into HR [24], from BR into SRII [25] and from SRI and SRII into BR [26,27]).

These classical rhodopsins have become a model both of membrane proteins and photoactive proteins, because of several characteristics (e.g., they are relatively small membrane proteins consisting of only ~250 amino acid residues with a single retinal chromophore and their photoabsorption abilities allow to control the biological activity by light at high temporal (~ tens of fs) and spatial (~ tens of nm) resolutions). In fact, high resolution tertiary structures of BR, HR and SRII, both in the ground states and in many of the photointermediates during the photocycle, have been reported by X-ray crystallography, electron microscopy and NMR spectroscopy [1,28]. Time-resolved pump-probe spectroscopy was utilized to investigate the structure and structural changes both in the excited states and in photointermediates in a wide range of time frames from femtoseconds to seconds [1,29,30]. The detailed structures and structural changes, both of the protein moiety and the retinal chromophore, have also been extensively analyzed by vibrational spectroscopies and theoretical studies [1,3134].

Thus, the archael rhodopsins have become models for the simplest and most essential features both of photoactive proteins and of membrane-embedded proteins.

New aspects for microbial rhodopsins

Wide distribution in organisms

Although a large number of genes encoding microbial type-1 opsins have been identified in archaea, no opsin gene from bacteria or eukarya has been identified until 1997. In 1997, two groups have made a breakthrough regarding the distribution of microbial rhodopsins. They have identified microbial rhodopsin homologs from fungi [35,36]. In 1999, Borkovich and coworkers have found a novel gene that encodes an opsin from the eukaryotic filamentous fungus Neurospora crassa, and named it Neurospora rhodopsin (NR) (Fig. 3) [37,38]. Since then, hundreds of new rhodopsin have been found between 1997 and today. For instance, two light-driven proton pumps, Proteorhodopsin (PR) and Xanthorhodopsin (XR), have been found in the eubacterial marine bacterioplankton in 2000 [39] and in the halophile eubacterial Salinibacter ruber in 2005 [40], respectively (Fig. 3). Interestingly, XR possesses a secondary chromophore called salinixanthin (SX) as a light-harvesting antenna in the native membrane, and the efficiency of energy transfer from SX to retinal has been calculated as approximately 45% [40]. Those findings encouraged us to seek unidentified microbial rhodopsins from nature. Regarding the contributions of our group, we found (or characterized) several novel microbial rhodopsins, including highly stable SRI homologs from the eubacterium Salinibacter ruber (SrSRI) in 2008 [41] and from the archaeon Haloarcula vallismortis (HvSRI) in 2010 [42], on the basis of progress in environmental genomics. Further, we identified Middle rhodopsin (MR) from the archaeon Haloquadratum walsbyi as an evolutionary intermediate between BR and SRII in 2011 [43] and Thermophilic rhodopsin (TR) as a thermally stable light-driven proton pump derived from a thermophilic organism in 2013 [44]. Please see extensive reviews for the contributions of other groups [1,2,4,5]. These newly discovered microbial type-1 rhodopsins are shown in Figure 3 and are also summarized in Table 1 along with some of their photochemical and biological properties.

Figure 3.

Figure 3

The wide distribution of microbial rhodopsins in all three domains of life. By 1999, type-1 rhodopsins have only been described in halophilic archaea. The four classical rhodopsins (BR, HR, SRI and SRII) are shown as boxes with white letters. After 1999, environmental genomics revealed that microbial rhodopsins are broadly distributed among bacteria (green) and eukarya (blue) in addition to archaea (red).

Table 1.

Examples of newly discovered microbial rhodopsins

Opsin type Year identified Origin λmax [nm] Function
NRa 1999 Neurospora crassa 534 unknown
PRb 2000 Marine bacterioplankton 520 H+ pump
ChRc 2002 Chlamydomonas reinhardtii 505 Cation (H+, Na+, etc) channel
ASRd 2003 Anabaena (Nostoc) sp. PCC7120 543 Transcriptional regulator
XRe 2005 Salinibacter ruber 560 H+ pump
SrSRIf 2008 Salinibacter ruber 558 Phototaxis sensor
MRg 2011 Haloquadratum walsbyi 485 unknown
TRh 2013 Thermus thermophilus 530 H+ pump
KR2i 2013 Krokinobacter eikastus 524 Na+ pump
ACR2j 2015 Guillardia theta 470 Anion (Cl, Br, etc) channel
a

ref [37]

b

ref [39]

c

ref [49]

d

ref [55]

e

ref [40]

f

ref [41]

g

ref [43]

h

ref [44]

i

ref [46]

j

ref [50].

Thus today, a variety of type-1 rhodopsin genes have been identified from all domains of life (i.e., archaea, eubacteria and eukarya) and the number of genes is up to tens of thousands, indicating the great physiological significance of the widely distributed microbial rhodopsins for organisms (Fig. 3) [45].

Rich functional diversity

The wide distribution of microbial rhodopsins has also revealed their rich functional diversity (Fig. 4). It is well-known that there are two types of membrane-embedded ion transport machineries in nature. Ion pumps generate an electrochemical potential by energy-coupled active ion transportation, while one of the functions of ion channels is to produce an action potential by passive ion transportation. By 1999, two types of ion pumping rhodopsins, for H+ and Cl, are known to exist only in archaea [3]. The Cl pumping rhodopsins can also transport other anions such as Br and I [16], whereas, only proton (H+) can be a substrate ion but not other cations including Na+, K+ and Rb+. This is reasonable because of a characteristic property of H+. It is known that H+ can be rapidly transferred through several functional groups, including the OH group of the water molecule by the Grotthuss mechanism, but direct ion translocation is required for other cations. However, in 2013, a light-driven Na+ pumping rhodopsin (KR2) was first identified from the marine bacterium Krokinobacter eikastus (Fig. 3 and 4a) [46]. That unique ion transport mechanism was characterized by X-ray crystallography in 2015 [47,48]. In short, no binding of Na+ to the vicinity of the Schiff base under the unphotolyzed state is observed for the Na+ pumping rhodopsin. For ion channels, in 2002 and 2003, two light-gated cation channels, Channelrhodopsin-1 (ChR1) and Channelrhodopsin-2 (ChR2), were found in the eukaryotic green alga Chlamydomonas reinhardtii [49]. In 2015, two other light-gated anion channels, anion channelrhodopsin-1 (ACR1) and anion channelrhodopsin-2 (ACR2), were found in the eukaryotic cryptophyte Guillardia theta (Fig. 3 and 4a) [50]. Thus, the control of ion concentrations by the ion transporters is one of the most essential functions of microbial rhodopsins. For instance, the discovery of PR indicated that a previously unsuspected mode of bacterially mediated light-driven ATP production commonly occurs in oceanic surface waters worldwide [51]. How can pumps and channels function differently? In 2015, we succeeded in the functional conversion of an H+ pump Archaerhodopsin-3 (AR3) from the archaeon Halorubrum sodomense to an H+ channel by replacing only three amino acid residues around the retinal chromophore [52]. That result indicates that essential differences between pumps and channels in the rhodopsin family are much smaller than previously imagined.

Figure 4.

Figure 4

Multitalented microbial rhodopsins. The type-1 microbial rhodopsins are roughly categorized as ion transporters (i.e., cation pump, anion pump and ion channel) and as sensors. (a) For ion transporters, the number of substrates (X, Y and Z) greatly increased in the early 21st century. (b) For sensors and coenzymes, the photosensory function was also extended from only the regulation of kinases to the control of cyclases, transcriptional regulation and depolarization of the plasma membrane that accounts for the phototactic behaviors of the prokaryote Chlamydomonas reinhardtii.

In addition to ion transportation, rhodopsins are also involved in photoreception for signal transduction (Fig. 4b) [53]. As shown in Figure 2, SRI and SRII form signaling complex with their cognate transducer proteins (HtrI for SRI and HtrII for SRII). The complexes are responsible for positive and negative phototaxis via the control of kinase activity inside cells [53]. As the other type of sensory signal transduction, depolarization by the light-gated cation transport activity of ChRs is utilized to control the motility of cells through regulation of the flagellar motor apparatus [54]. As a novel type of photoreceptor, Spudich and coworkers identified Anabaena sensory rhodopsin (ASR) from the cyanobacterium Anabaena (Nostoc) sp. PCC7120 in 2003 [55] and we demonstrated its function as a photo-dependent transcriptional regulator in 2012 [56]. As other functions, a photochromic histidine kinase rhodopsin (HKR) [57] and the rhodopsin-guanylyl cyclase (RhGC) [58] have been identified in 2012 and 2015, respectively. It is noteworthy that the biological function(s) of many microbial rhodopsins, including NR and MR, is still unclear (Fig. 3). In other words, the expanded biological roles of microbial rhodopsins should become obvious in the future.

Great potential for optogenetics

In 2005, Boyden and coworkers made a breakthrough regarding a new technology named “optogenetics” (Fig. 5) [59]. In that study, they expressed ChR2 in mammalian cells to excite neurons by cation influx upon photoillumination. In 2007 and 2010, the same group succeeded in light-induced neural silencing using a Cl pumping rhodopsin HR from the archaeon Natronomonas pharaonis (NpHR) [60] and an H+ pumping rhodopsin AR3 [61], respectively. Upon photoexcitation, the ion pumps induce a hyperpolarization across the membrane in a nerve cell, results in its neural silencing. Thus, these ion transport machineries are biotechnologically utilized as a molecular switch to control neural activity by light (Fig. 5a). It is noteworthy that in 2012, Cohen and coworkers utilized the endogenous fluorescence of AR3 to monitor the single action potential in cultured rat hippocampal neurons [62]. Since then, they succeeded in directly measuring the membrane voltage at the millivolt-level (Fig. 5b) [63].

Figure 5.

Figure 5

Optogenetic applications. (a) The photo-activation of ion transporting rhodopsins induces a hyperpolarization used for neural silencing and a depolarization used for neural activation both in vivo and in vitro. (b) The genetically modified H+ pump AR3 is utilized to measure the membrane potential in vivo. (c) The color variants of ion transporting rhodopsins allow optogenetics control in a wide range of wavelengths of light. (d) One of the sensory rhodopsins, Anabaena sensory rhodopsin (ASR), can be utilized as a tool for arbitrary protein expression in vivo regulated by visible light. (e) Chimeric proteins of Gloeobacter rhodopsin (GR) and bovine visual rhodopsin (Rh) are utilized to activate the trimeric G-protein.

As for luminescent and fluorescent proteins, the color variants of rhodopsins make it possible to use various wavelengths of visible light. For rhodopsins, the absorption maximum corresponds to the most probable transition from its ground to its excited state. All microbial rhodopsins reported are quite similar in their primary and tertiary structures, especially in the chromophore-binding site [8], which suggests that the absorption maximum is mainly controlled by the slight structural difference(s) of their side chain(s). Regarding the retinal chromophore, if the polyene chain possesses a planar conformation, a π-conjugation of the retinal becomes long (see Fig. 1a). A rotation of the β-ionone ring can, despite breaking the π-conjugation, easily occur in an isolated condition because of the steric repulsions between the methyl groups of the β-ionone ring at positions 1 and 5 and the hydrogens of the polyene chain at positions 7 and 8 (see Fig. 1a) [1]. To characterize this in the rhodopsins, we prepared a variety of rhodopsin mutants. As a result, we succeeded in developing blue-shifted color variants both of AR3 and ChR with a few mutations (~3) on the basis of rational design at atomic resolution, achieved through accurate molecular simulations, electrophysiology and X-ray crystallography [64,65]. The molecular simulation models and the crystal structure revealed the precisely designed conformational changes of the chromophore induced by combinatory mutations. The mutants shrink its π-conjugated system which produce large blue shifts (maximally 100 nm) of the absorption spectra (Fig. 5c). Of note, in the mutants, photosensitive ion transport activities are maintained. Thus, we successfully produced blue-shifted color variants both for neural silencing and excitation. It should be noted that some groups in USA and Germany have also succeeded in production of blue-shifted variants [6668].

In addition to the control of neural activity, the photo-activation of an intracellular signaling cascade is also useful, and is recognized as one aspect of optogenetics. In 2012, we demonstrated that ASR represses the transcription of the protein expression in the cytosol and that this is fully inhibited by the light activation of ASR [56]. The system provides an advantage in controlling the amount of protein at temporal/spatial resolution and in providing control of the timing, compared with other techniques (Fig. 5d). In the future, ASR might be a novel tool for optogenetics, next to the ion transporting rhodopsins. Another interesting and demanding target is the hetero-trimeric G-protein, which is activated by GPCR, because various signaling processes in mammalian cells are regulated by GPCR [10]. Although the activity is still low, Kandori and coworkers have reported that chimeric proteins between a type-1 proton pump rhodopsin from the eubacterium Gloeobacter violaceus (GR) and bovine visual type-2 rhodopsin (Rh) can activate a type of G-protein by light like native Rh (Fig. 5e) [69,70]. However, its relation to the optogenetics is still unclear. Thus, compared with the ion transporting rhodopsins, further progress is needed to apply sensory rhodopsins as optogenetic tools.

Future perspectives

In the 20th century, microbial rhodopsins were research models both for membrane-embedded proteins and for photoactive proteins. Subsequently, in the early 21st century, they have become a fundamental template for optogenetics technology. Progress has been continuously assisted by a variety of scientific approaches, including biophysics, biochemistry and molecular biology. Of note, the United Nations determined the year 2015 as the International Year of Light and Light-based Technologies (IYL 2015: http://www.light2015.org/Home.html). Because light is a powerful tool, both for observing and controlling physical, chemical and biological phenomena in a variety of samples with various spatial dimensions from the atom to the organism, we strongly believe that making maximum use of light will open the door for the next generation of rhodopsin research.

Acknowledgements

YS wishes to thank collaborators, especially Profs. Naoki Kamo, John Spudich and Hideki Kandori, for giving me the opportunity to study microbial rhodopsins extensively. Our original publications were supported by a Grant-in-Aid from the Japanese Ministry of Education, Science, Technology, Sports and Cultures. Support for this work was provided by the Foundation of the Promotion of Ion Engineering and Research Foundation for Opto-science and Technology. We also thank DASS Manuscript for the expert English language review.

Footnotes

Conflicts of Interest

All authors declare that they have no conflict of interest.

Author Contributions

M. K., and Y. S. prepared the figures and wrote the manuscript.

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