Abstract
A simple automated fluorometric method for the estimation of lactate and pyruvate is described. Analyses can be performed at 60/hour and can conveniently determine pyruvate and lactate in the ranges 0-5 mg. and 0-30 mg/100 ml. The methods give a high degree of analytical precision and good yields from recovery experiments.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Antonis A., Clark M., Pilkington T. R. A semiautomated fluorimetric method for the enzymatic determination of pyruvate, lactate, acetoacetate, and beta-hydroxybutyrate levels in plasma. J Lab Clin Med. 1966 Aug;68(2):340–356. [PubMed] [Google Scholar]
- GLOSTER J. A., HARRIS P. Observations on an enzymic method for the estimation of pyruvate in blood. Clin Chim Acta. 1962 Mar;7:206–211. doi: 10.1016/0009-8981(62)90011-6. [DOI] [PubMed] [Google Scholar]
- GREENGARD P. Determination of intermediary metabolites by enzymic fluorimetry. Nature. 1956 Sep 22;178(4534):632–634. doi: 10.1038/178632a0. [DOI] [PubMed] [Google Scholar]
- HOCHELLA N. J., WEINHOUSE S. AUTOMATED LACTIC ACID DETERMINATION IN SERUM AND TISSUE EXTRACTS. Anal Biochem. 1965 Feb;10:304–317. doi: 10.1016/0003-2697(65)90271-x. [DOI] [PubMed] [Google Scholar]
- HUCKABEE W. E. Control of concentration gradients of pyruvate and lactate across cell membranes in blood. J Appl Physiol. 1956 Sep;9(2):163–170. doi: 10.1152/jappl.1956.9.2.163. [DOI] [PubMed] [Google Scholar]
- LANDON J., FAWCETT J. K., WYNN V. Blood pyruvate concentration measured by a specific method in control subjects. J Clin Pathol. 1962 Nov;15:579–584. doi: 10.1136/jcp.15.6.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROBERTS N. R., KAPPHAHN J. I. The fluorometric measurement of pyridine nucleotides. J Biol Chem. 1957 Feb;224(2):1047–1064. [PubMed] [Google Scholar]
- MEISTER A. Reduction of alpha gamma-diketo and alpha-keto acids catalyzed by muscle preparations and by crystalline lactic dehydrogenase. J Biol Chem. 1950 May;184(1):117–129. [PubMed] [Google Scholar]
- Wynn V., Doar J. W. Some effects of oral contraceptives on carbohydrate metabolism. Lancet. 1966 Oct 1;2(7466):715–719. doi: 10.1016/s0140-6736(66)92978-3. [DOI] [PubMed] [Google Scholar]