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. 1968 Mar;21(2):175–178. doi: 10.1136/jcp.21.2.175

Technique for lymphocyte transformation

C R Pentycross 1,2
PMCID: PMC473724  PMID: 5697050

Abstract

Current techniques for lymphocyte transformation are evaluated and criticised. A simple technique, designed to meet these criticisms, is described in detail, with particular reference to lymphocyte separation and scoring methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRANDT L., BORJESON J., NORDEN A., OLSSON I. Separation of lymphocytes from peripheral blood by means of a glass wool column. A method for in vitro culture of lymphocytes. Acta Med Scand. 1962 Oct;172:459–462. doi: 10.1111/j.0954-6820.1962.tb07179.x. [DOI] [PubMed] [Google Scholar]
  2. CARSTAIRS K. The human small lymphocyte: its possible pluripotential quality. Lancet. 1962 Apr 21;1(7234):829–832. doi: 10.1016/s0140-6736(62)91841-x. [DOI] [PubMed] [Google Scholar]
  3. COULSON A. S., CHALMERS D. G. SEPARATION OF VIABLE LYMPHOCYTES FROM HUMAN BLOOD. Lancet. 1964 Feb 29;1(7331):468–469. doi: 10.1016/s0140-6736(64)90799-8. [DOI] [PubMed] [Google Scholar]
  4. Czerski P., Szmigielski S., Litwin J. Simple methods for obtaining purified suspensions of lymphocytes. Vox Sang. 1966 Nov-Dec;11(6):734–737. doi: 10.1111/j.1423-0410.1966.tb04278.x. [DOI] [PubMed] [Google Scholar]
  5. HUNGERFORD D. A., DONNELLY A. J., NOWELL P. C., BECK S. The chromosome constitution of a human phenotypic intersex. Am J Hum Genet. 1959 Sep;11:215–236. [PMC free article] [PubMed] [Google Scholar]
  6. LING N. R., HUSBAND E. M. SPECIFIC AND NON-SPECIFIC STIMULATION OF PERIPHERAL LYMPHOCYTES. Lancet. 1964 Feb 15;1(7329):363–365. doi: 10.1016/s0140-6736(64)92102-6. [DOI] [PubMed] [Google Scholar]
  7. MOYNIHAN P. C., JACKSON J. F., HARDY J. D. LYMPHOCYTE TRANSFORMATION AS AN IN-VITRO HISTOCOMPATIBILITY TEST. Lancet. 1965 Feb 27;1(7383):453–455. doi: 10.1016/s0140-6736(65)91587-4. [DOI] [PubMed] [Google Scholar]
  8. NOWELL P. C. Differentiation of human leukemic leukocytes in tissue culture. Exp Cell Res. 1960 Mar;19:267–277. doi: 10.1016/0014-4827(60)90007-0. [DOI] [PubMed] [Google Scholar]
  9. SCHREK R., STEFANI S. RADIORESISTANCE OF PHYTOHEMAGGLUTININ- TREATED NORMAL AND LEUKEMIC LYMPHOCYTES. J Natl Cancer Inst. 1964 Feb;32:507–521. [PubMed] [Google Scholar]

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