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. 2014 Feb 27;8(1-2):168–178. doi: 10.1002/jbio.201300203

A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue

Hugh Sparks 1,✉, Sean Warren 1,2, Joana Guedes 3, Nagisa Yoshida 3, Tze Choong Charn 4, Nadia Guerra 3, Taranjit Tatla 4, Christopher Dunsby 1, Paul French 1
PMCID: PMC4737404  PMID: 24573953

Abstract

Fluorescence lifetime imaging (FLIM) has previously been shown to provide contrast between normal and diseased tissue. Here we present progress towards clinical and preclinical FLIM endoscopy of tissue autofluorescence, demonstrating a flexible wide‐field endoscope that utilised a low average power blue picosecond laser diode excitation source and was able to acquire ∼mm‐scale spatial maps of autofluorescence lifetimes from fresh ex vivo diseased human larynx biopsies in ∼8 seconds using an average excitation power of ∼0.5 mW at the specimen. To illustrate its potential for FLIM at higher acquisition rates, a higher power mode‐locked frequency doubled Ti:Sapphire laser was used to demonstrate FLIM of ex vivo mouse bowel at up to 2.5 Hz using 10 mW of average excitation power at the specimen. (© 2014 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim)

Keywords: FLIM, endoscope, autofluorescence, lifetime, fibre, flexible, lifetime, instrument

Supporting information

Author biographies

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Author biographies

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