Skip to main content
PMC home page
Thorax logoLink to Thorax
. 1990 Apr;45(4):276–280. doi: 10.1136/thx.45.4.276

Effect of bacterial products on neutrophil migration in vitro.

G Ras 1, R Wilson 1, H Todd 1, G Taylor 1, P Cole 1
PMCID: PMC473772  PMID: 2113319

Abstract

Chronic bronchial inflammation is associated with migration of large numbers of granulocytes into the bronchial tree. A study was designed to find out whether products of bacteria commonly isolated in chronic bronchial infection stimulate neutrophil migration in vitro. Neutrophils from healthy donors were studied by a modified Boyden chamber technique. Bacterial culture filtrates stimulated neutrophil migration over a wide dilution range and the chemotactic activity was heat stable. Culture filtrates derived from Pseudomonas aeruginosa, Streptococcus pneumoniae, and Haemophilus influenzae were significantly chemokinetic and directionally chemotactic, whereas filtrates from Staphylococcus aureus were only chemotactic. Gel filtration of S aureus and P aeruginosa culture filtrates yielded high, medium, and low molecular weight fractions showing chemotactic activity. S pneumoniae and H influenzae yielded fractions with only low molecular weight chemotactic activity. Neutrophil chemotactic responses, occurring in response to all bacterial species tested, appear to represent a defence mechanism by the host. Chemoattractant activity may, however, contribute to bronchial damage mediated by products released from continuously attracted, activated neutrophils.

Full text

PDF
276

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barton A. D., Ryder K., Lourenço R. V., Dralle W., Weiss S. G. Inflammatory reaction and airway damage in cystic fibrosis. J Lab Clin Med. 1976 Sep;88(3):423–426. [PubMed] [Google Scholar]
  2. Currie D. C., Saverymuttu S. H., Peters A. M., Needham S. G., George P., Dhillon D. P., Lavender J. P., Cole P. J. Indium-111-labelled granulocyte accumulation in respiratory tract of patients with bronchiectasis. Lancet. 1987 Jun 13;1(8546):1335–1339. doi: 10.1016/s0140-6736(87)90647-7. [DOI] [PubMed] [Google Scholar]
  3. Falk W., Goodwin R. H., Jr, Leonard E. J. A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration. J Immunol Methods. 1980;33(3):239–247. doi: 10.1016/0022-1759(80)90211-2. [DOI] [PubMed] [Google Scholar]
  4. Harvey R. L., Kronvall G., Troup G. M., Anderson R. E., Williams R. C., Jr Chemotaxis of polymorphonuclear leukocytes by protein A of the staphylococcus. Proc Soc Exp Biol Med. 1970 Nov;135(2):453–456. doi: 10.3181/00379727-135-35073. [DOI] [PubMed] [Google Scholar]
  5. LIEBERMAN J., KURNICK N. B. Proteolytic enzyme activity and the role of desoxyribose nucleic acid (DNA) in cystic fibrosis sputum. Pediatrics. 1963 Jun;31:1028–1032. [PubMed] [Google Scholar]
  6. Lynn M., Sensakovic J. W., Bartell P. F. In vivo distribution of Pseudomonas aeruginosa slime glycolipoprotein: association with leukocytes. Infect Immun. 1977 Jan;15(1):109–114. doi: 10.1128/iai.15.1.109-114.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Maestrelli P., Tsai J. J., Cromwell O., Kay A. B. The identification and partial characterization of a human mononuclear cell-derived neutrophil chemotactic factor apparently distinct from IL-1, IL-2, GM-CSF, TNF and IFN-gamma. Immunology. 1988 Jun;64(2):219–225. [PMC free article] [PubMed] [Google Scholar]
  8. Nelson R. D., Herron M. J. Chemotaxis and motility of lung phagocytes. Semin Respir Infect. 1986 Jun;1(2):79–88. [PubMed] [Google Scholar]
  9. Roberts D. E., Cole P. Use of selective media in bacteriological investigation of patients with chronic suppurative respiratory infection. Lancet. 1980 Apr 12;1(8172):796–797. doi: 10.1016/s0140-6736(80)91295-7. [DOI] [PubMed] [Google Scholar]
  10. Roberts D. E., Higgs E., Cole P. J. Selective medium that distinguishes Haemophilus influenzae from Haemophilus parainfluenzae in clinical specimens: its value in investigating respiratory sepsis. J Clin Pathol. 1987 Jan;40(1):75–76. doi: 10.1136/jcp.40.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Saverymuttu S. H., Peters A. M., Lavender J. P., Pepys M. B., Hodgson H. J., Chadwick V. S. Quantitative fecal indium 111-labeled leukocyte excretion in the assessment of disease in Crohn's disease. Gastroenterology. 1983 Dec;85(6):1333–1339. [PubMed] [Google Scholar]
  12. Scharmann W. Cytotoxic effects of leukocidin from Pseudomonas aeruginosa on polymorphonuclear leukocytes from cattle. Infect Immun. 1976 Mar;13(3):836–843. doi: 10.1128/iai.13.3.836-843.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scharmann W. Interaction of purified leukocidin from Pseudomonas aeruginosa with Bovine polymorphonuclear leukocytes. Infect Immun. 1976 Apr;13(4):1046–1053. doi: 10.1128/iai.13.4.1046-1053.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Snyderman R., Shin H. S., Phillips J. K., Gewurz H., Mergenhagen S. E. A neutrophil chemotatic factor derived from C'5 upon interaction of guinea pig serum with endotoxin. J Immunol. 1969 Sep;103(3):413–422. [PubMed] [Google Scholar]
  15. Stockley R. A., Hill S. L., Morrison H. M., Starkie C. M. Elastolytic activity of sputum and its relation to purulence and to lung function in patients with bronchiectasis. Thorax. 1984 Jun;39(6):408–413. doi: 10.1136/thx.39.6.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stockley R. A., Shaw J., Hill S. L., Burnett D. Neutrophil chemotaxis in bronchiectasis: a study of peripheral cells and lung secretions. Clin Sci (Lond) 1988 Jun;74(6):645–650. doi: 10.1042/cs0740645. [DOI] [PubMed] [Google Scholar]
  17. Suter S., Schaad U. B., Roux L., Nydegger U. E., Waldvogel F. A. Granulocyte neutral proteases and Pseudomonas elastase as possible causes of airway damage in patients with cystic fibrosis. J Infect Dis. 1984 Apr;149(4):523–531. doi: 10.1093/infdis/149.4.523. [DOI] [PubMed] [Google Scholar]
  18. Sykes D. A., Wilson R., Greenstone M., Currie D. C., Steinfort C., Cole P. J. Deleterious effects of purulent sputum sol on human ciliary function in vitro: at least two factors identified. Thorax. 1987 Apr;42(4):256–261. doi: 10.1136/thx.42.4.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walker W. S., Barlet R. L., Kurtz H. M. Isolation and partial characterization of a staphylococcal leukocyte cytotaxin. J Bacteriol. 1969 Mar;97(3):1005–1008. doi: 10.1128/jb.97.3.1005-1008.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ward P. A., Lepow I. H., Newman L. J. Bacterial factors chemotactic for polymorphonuclear leukocytes. Am J Pathol. 1968 Apr;52(4):725–736. [PMC free article] [PubMed] [Google Scholar]
  21. Wardlaw A. J., Moqbel R., Cromwell O., Kay A. B. Platelet-activating factor. A potent chemotactic and chemokinetic factor for human eosinophils. J Clin Invest. 1986 Dec;78(6):1701–1706. doi: 10.1172/JCI112765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wilkinson P. C. Leukocyte locomotion and chemotaxis: effects of bacteria and viruses. Rev Infect Dis. 1980 Mar-Apr;2(2):293–318. doi: 10.1093/clinids/2.2.293. [DOI] [PubMed] [Google Scholar]
  23. Wilson R. Secondary ciliary dysfunction. Clin Sci (Lond) 1988 Aug;75(2):113–120. doi: 10.1042/cs0750113. [DOI] [PubMed] [Google Scholar]

Articles from Thorax are provided here courtesy of BMJ Publishing Group

RESOURCES