. Author manuscript; available in PMC: 2017 Feb 2.

Published in final edited form as: Biochemistry. 2016 Jan 15;55(4):697–703. doi: 10.1021/acs.biochem.5b01325

Table 1.

Steady-State Kinetic Parameters for LHNO^*

Substrate	k_cat, s⁻¹	k_cat/K_amine, mM⁻¹s⁻¹	k_cat/K_O2, mM⁻¹s⁻¹	K_amine, mM	K_O2, mM
(S)-6-hydroxynicotine	78 ± 10 (30 ± 1) ^a	600 ± 260 (730 ± 100)^a	270 ± 60	0.13 ± 0.03 (0.042 ± 0.007)^a	0.29 ± 0.10
(S)-6-hydroxynornicotine	70 ± 4 (16 ± 1)^a	370 ± 50 (480 ± 90)^a	150 ± 10	0.20 ± 0.03 (0.064 ± 0.014)^a	0.46 ± 0.07
(S)-nicotine^a	0.033 ± 0.002^a	0.042 ± 0.007	ND^b	0.80 ± 0.16^a	ND
(S)-4-(1-methyl pyrrolidine-2-yl)phenol^a	0.044 ± 0.002^a	0.069± 0.008	ND	0.64 ± 0.10^a	ND

Conditions: 0.1 M Hepes (pH 8.0), 0.1 M NaCl, 25 °C.

Apparent value determined by varying the concentration of the amine with 250 µM oxygen and fitting the data to the Michaelis-Menten equation.

ND, not determined