Abstract
Three turbidometric methods and one method using ultraviolet spectrophotometry for estimating total cerebrospinal fluid protein have been examined. The necessity for preliminary dialysis renders the ultraviolet method unsuitable for routine use.
The turbidometric method of Meulemans (1960) using a sulphosalicylic acid-sodium sulphate precipitating fluid is better than a method using sulphosalicylic acid alone which is affected by the albumin-globulin ratio, and has a greater sensitivity and better reproducibility than a method using trichloracetic acid as a precipitant. Turbidity may be measured with a spectrophotometer or an MRC grey wedge photometer with human or bovine albumin as a standard. This method deserves wider acceptance.
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Selected References
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- ALEXANDER J. G., PARKES J. The estimation of protein in the cerebrospinal fluid using the M.R.C. photometer. J Clin Pathol. 1959 Jan;12(1):91–91. doi: 10.1136/jcp.12.1.91-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRACKENRIDGE C. J. Cerebrospinal fluid protein fractions in health and disease. J Clin Pathol. 1962 May;15:206–210. doi: 10.1136/jcp.15.3.206. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUTHIE E. S. The action of fibrinogen on certain pathogenic cocci. J Gen Microbiol. 1955 Oct;13(2):383–393. doi: 10.1099/00221287-13-2-383. [DOI] [PubMed] [Google Scholar]
- FERREIRA H. C., MURAT L. G. An immunological method for demonstrating fibrin degradation products in serum and its use in the diagnosis of fibrinolytic states. Br J Haematol. 1963 Jul;9:299–310. doi: 10.1111/j.1365-2141.1963.tb06554.x. [DOI] [PubMed] [Google Scholar]
- GOLDFARB A. R., SAIDEL L. J., MOSOVICH E. The ultraviolet absorption spectra of proteins. J Biol Chem. 1951 Nov;193(1):397–404. [PubMed] [Google Scholar]
- HENRY R. J., SEGALOVE M., SOBEL C. Turbidimetric determination of proteins with sulfosalicylic and trichloracetic acids. Proc Soc Exp Biol Med. 1956 Aug-Sep;92(4):748–751. doi: 10.3181/00379727-92-22601. [DOI] [PubMed] [Google Scholar]
- MEULEMANS O. Determination of total protein in spinal fluid with sulphosalicylic acid and trichloroacetic acid. Clin Chim Acta. 1960 Sep;5:757–761. doi: 10.1016/0009-8981(60)90020-6. [DOI] [PubMed] [Google Scholar]
- Schriever H., Gambino S. R. Protein turbidity produced by trichloracetic acid and sulfosalicylic acid at varying temperatures and varying ratios of albumin and globulin. Am J Clin Pathol. 1965 Dec;44(6):667–672. doi: 10.1093/ajcp/44.6.667. [DOI] [PubMed] [Google Scholar]
- TOMBS M. P., SOUTER F., MACLAGAN N. F. The spectrophotometric determination of protein at 210 millimicrons. Biochem J. 1959 Sep;73:167–171. doi: 10.1042/bj0730167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WADDELL W. J. A simple ultraviolet spectrophotometric method for the determination of protein. J Lab Clin Med. 1956 Aug;48(2):311–314. [PubMed] [Google Scholar]
- YEOMAN W. B. The estimation of protein in cerebrospinal fluid using the M.R.C. photometer. J Clin Pathol. 1955 Aug;8(3):252–252. doi: 10.1136/jcp.8.3.252. [DOI] [PMC free article] [PubMed] [Google Scholar]