Abstract
An immunofluorescence technique for demonstrating Treponema pallidum in fixed films made from suspected chancre exudate is described. The method used is basically a reversal of the fluorescent treponemal antibody absorption test. It makes use of the fact that by using a syphilitic serum from which group antibody has been absorbed by an extract of Reiter treponemes, leaving only specific antibody, Treponema pallidum can be indentified. Neither the non-pathogenic genital treponemes nor mouth treponemes showed any fluorescence when tested by the technique described.
All the reagents used in the test are available commercially, thus placing it within the scope of any laboratory with suitable fluorescence equipment.
In the present state of knowledge of the treponematoses, this technique would seen to be the most accurate means of diagnosing primary syphilis because it permits the specific identification of Treponema pallidum in chancre exudate. It has the advantage over the dark-ground method that fixed films can be sent to the laboratory.
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Selected References
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- DEACON W. E., HUNTER E. F. Treponemal antigens as related to identification and syphilis serology. Proc Soc Exp Biol Med. 1962 Jun;110:352–356. doi: 10.3181/00379727-110-27515. [DOI] [PubMed] [Google Scholar]
- Garner M. F. Early primary syphilis--a fluorescent test to replace dark-ground examination of chancre exudate. Med J Aust. 1967 Jul 29;2(5):199–200. doi: 10.5694/j.1326-5377.1967.tb97713.x. [DOI] [PubMed] [Google Scholar]
- HUNTER E. F., DEACON W. E., MEYER P. E. AN IMPROVED FTA TEST FOR SYPHILIS, THE ABSORPTION PROCEDURE (FTA-ABS). Public Health Rep. 1964 May;79:410–412. [PMC free article] [PubMed] [Google Scholar]
- KELLOGG D. S., Jr, DEACON W. E. A NEW RAPID IMMUNOFLUORESCENT STAINING TECHNIQUE FOR IDENTIFICATION OF TREPONEMA PALLIDUM AND NEISSERIA GONORRHOEAE. Proc Soc Exp Biol Med. 1964 Apr;115:963–965. doi: 10.3181/00379727-115-29090. [DOI] [PubMed] [Google Scholar]