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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1968 Sep;21(5):611–615. doi: 10.1136/jcp.21.5.611

Intravenous iron-dextran: studies on unsaturated iron-binding capacity

J S G Cox 1, G F Moss 1, I Bremner 1,1, Janet Reason 1
PMCID: PMC473873  PMID: 5697365

Abstract

A method is described for measuring the plasma unsaturated iron-binding capacity in the presence of very high concentrations of iron as iron-dextran. The procedure utilizes 59Fe to label the apotransferrin with subsequent separation of ionic iron from transferrin-bound iron on an ion exchange or Sephadex G.25 column.

The unsaturated iron-binding capacity has been measured in rabbits and dogs after intravenous injection of iron-dextran and in human subjects after total dose infusion of iron-dextran. No evidence of saturation of the unsaturated iron-binding capacity was found even when the plasma iron values were greater than 40,000 μg Fe/100 ml.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARBER A., DEMPSTER C., ANDERSON N. G. A gel filtration method for studies on protein iron-binding. Clin Chim Acta. 1963 Jan;8:143–145. doi: 10.1016/0009-8981(63)90211-0. [DOI] [PubMed] [Google Scholar]
  2. BIRDSALL N. J., KOK D., WILD F. DETERMINATION OF THE SERUM IRON-BINDING CAPACITY. J Clin Pathol. 1965 Jul;18:453–455. doi: 10.1136/jcp.18.4.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bates G. W., Billups C., Saltman P. The kinetics and mechanism of iron (3) exchange between chelates and transferrin. I. The complexes of citrate and nitrilotriacetic acid. J Biol Chem. 1967 Jun 25;242(12):2810–2815. [PubMed] [Google Scholar]
  4. Cox J. S., King R. E., Reynolds G. F. Valency investigations of iron dextran ("Imferon"). Nature. 1965 Sep 11;207(5002):1202–1203. doi: 10.1038/2071202a0. [DOI] [PubMed] [Google Scholar]
  5. DAWSON D. W., GOLDTHORP W. O., SPENCER D. PARENTERAL IRON THERAPY IN PREGNANCY. J Obstet Gynaecol Br Commonw. 1965 Feb;72:89–93. doi: 10.1111/j.1471-0528.1965.tb01378.x. [DOI] [PubMed] [Google Scholar]
  6. FIELDING J., SMITH G. M. The haemolytic activity of an iron carbohydrate complex. J Clin Pathol. 1963 Jan;16:12–17. doi: 10.1136/jcp.16.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KOK D., WILD F. Serum iron determination. J Clin Pathol. 1960 May;13:241–245. doi: 10.1136/jcp.13.3.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ricketts C. R., Cox J. S., Fitzmaurice C., Moss G. F. The iron-dextran complex. Nature. 1965 Oct 16;208(5007):237–239. doi: 10.1038/208237a0. [DOI] [PubMed] [Google Scholar]

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