Abstract
Analysis of blood group antibodies on Sephadex G-200 columns is described.
The position of blood group antibodies when whole serum containing them is subjected to gel filtration is studied and compared with the position of the γ-globulins and of standard blood group antibodies.
The technique on its own cannot be used to identify the immunoglobulin nature of the antibody but is very useful when employed in conjunction with the antiglobulin test using specific antisera and with other methods.
The technique is rapid, simple, and reliable.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adinolfi M., Mollison P. L., Polley M. J., Rose J. M. Gamma-A-blood group antibodies. J Exp Med. 1966 May 1;123(5):951–967. doi: 10.1084/jem.123.5.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Castanedo J. P., Williams R. C., Jr Anticomplementary activity of sera from patients with connective tissue disease and normal subjects. J Lab Clin Med. 1967 Feb;69(2):217–228. [PubMed] [Google Scholar]
- FAHEY J. L., MCKELVEY E. M. QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES. J Immunol. 1965 Jan;94:84–90. [PubMed] [Google Scholar]
- Ishizaka K., Ishizaka T., Lee E. H., Fudenberg H. Immunochemical properties of human gamma-A isohemagglutinin. I. Comparisons with gamma-G and gamma-M-globulin antibodies. J Immunol. 1965 Aug;95(2):197–208. [PubMed] [Google Scholar]
- KILLANDER J., HOGMAN C. F. Fractionation of human blood group antibodies by gel filtration. Scand J Clin Lab Invest. 1963;15 (Suppl 69):130–138. [PubMed] [Google Scholar]
- KOCHWA S., ROSENFIELD R. E. IMMUNOCHEMICAL STUDIES OF THE RH SYSTEM. I. ISOLATION AND CHARACTERIZATION OF ANTIBODIES. J Immunol. 1964 May;92:682–692. [PubMed] [Google Scholar]
- POLLEY M. J., ADINOLFI M., MOLLISON P. L. SEROLOGICAL CHARACTERISTICS OF ANTI-A RELATED TO TYPE OF ANTIBODY PROTEIN (7 S GAMMA OR 19 S GAMMA). Vox Sang. 1963 Jul-Aug;8:385–409. doi: 10.1111/j.1423-0410.1963.tb04159.x. [DOI] [PubMed] [Google Scholar]
- STRATTON F., GUNSON H. H., RAWLINSON V. I. The preparation and uses of antiglobulin reagents with special reference to complement-fixing blood group antibodies. Transfusion. 1962 May-Jun;2:135–149. doi: 10.1111/j.1537-2995.1962.tb00212.x. [DOI] [PubMed] [Google Scholar]