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. 1969 May;22(3):263–268. doi: 10.1136/jcp.22.3.263

Serotyping and the Dienes reaction on Proteus mirabilis from hospital infections

J de Louvois 1,1
PMCID: PMC474056  PMID: 4891480

Abstract

The serotype of 320 strains of Proteus mirabilis from clinical material was determined. Using 20 O antisera and four H antisera 61% of strains could be fully identified and 90% partially identified. A large number of serotypes were recognized but no difference was found between the serotype of organisms infecting the urinary tract and those from other infections. Biochemically identical organisms found in the same ward generally differed in serology. Proteus mirabilis was isolated from the faeces of 84·5% of 84 patients with urinary infection and from none of 20 normal controls. By serology and the Dienes test 61% of the organisms isolated from the urine and faeces of a single patient were identical, indicating that infection arose from the intestine.

Most groups of serologically identical strains could, by the Dienes test, be further divided into a number of subtypes indicating that the strains were different and that cross infection had not been responsible for their spread. With three serological groups, however, the majority of strains belonged to a single Dienes type and it was concluded that these organisms had been spread from a common reservoir or carrier.

Because of the unreliability of the Dienes test when carried out on random organisms it is suggested that reliable results can only be obtained by combining the Dienes test with serotyping.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AYLIFFE G. A., LOWBURY E. J., HAMILTON J. G., SMALL J. M., ASHESHOV E. A., PARKER M. T. HOSPITAL INFECTION WITH PSEUDOMONAS AERUGINOSA IN NEUROSURGERY. Lancet. 1965 Aug 21;2(7408):365–368. doi: 10.1016/s0140-6736(65)90343-0. [DOI] [PubMed] [Google Scholar]
  2. BARBER M., WATERWORTH P. M. ANTIBIOTIC SENSITIVITY OF PROTEUS SPECIES. J Clin Pathol. 1964 Jan;17:69–74. doi: 10.1136/jcp.17.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BELYAVIN G. Cultural and serological phases of Proteus vulgaris. J Gen Microbiol. 1951 Feb;5(1):197–207. doi: 10.1099/00221287-5-1-197. [DOI] [PubMed] [Google Scholar]
  4. BROOKE M. S. The biochemical properties of Klebsiella strains with new capsular antigens. Acta Pathol Microbiol Scand. 1951;28(4):328–337. doi: 10.1111/j.1699-0463.1951.tb03698.x. [DOI] [PubMed] [Google Scholar]
  5. EDWARDS P. R., FIFE M. A. Capsule types of Klebsiella. J Infect Dis. 1952 Jul-Aug;91(1):92–104. doi: 10.1093/infdis/91.1.92. [DOI] [PubMed] [Google Scholar]
  6. FINLAND M. Treatment of pneumonia and other serious infections. N Engl J Med. 1960 Aug 4;263:207–221. doi: 10.1056/NEJM196008042630501. [DOI] [PubMed] [Google Scholar]
  7. France D. R., Markham N. P. Epidemiological aspects of Proteus infections with particular reference to phage typing. J Clin Pathol. 1968 Jan;21(1):97–102. doi: 10.1136/jcp.21.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gillies R. R., Govan J. R. Typing of Pseudomonas pyocyanea by pyocine production. J Pathol Bacteriol. 1966 Apr;91(2):339–345. doi: 10.1002/path.1700910207. [DOI] [PubMed] [Google Scholar]
  9. HABS I. Untersuchungen über die O-Antigene von Pseudomonas aeruginosa. Z Hyg Infektionskr. 1957;144(3):218–228. [PubMed] [Google Scholar]
  10. Huang C. T. Multitest media for rapid identification of Proteus species with notes on biochemical reactions of strains isolated from urine and pus. J Clin Pathol. 1966 Sep;19(5):438–442. doi: 10.1136/jcp.19.5.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KIPPAZ P. W. A study of Proteus infections in a male urological ward. J Clin Pathol. 1957 Aug;10(3):211–214. doi: 10.1136/jcp.10.3.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LANYI B. Serological typing of Proteus strains from infantile enteritis and other sources. Acta Microbiol Acad Sci Hung. 1956;3(4):417–428. [PubMed] [Google Scholar]
  13. PERCIVAL A., BIRUMFITT W., DELOUVOIS J. SERUM-ANTIBODY LEVELS AS AN INDICATION OF CLINICALLY INAPPARENT PYELONEPHRITIS. Lancet. 1964 Nov 14;2(7368):1027–1033. doi: 10.1016/s0140-6736(64)90988-2. [DOI] [PubMed] [Google Scholar]
  14. STORY P. Proteus infections in hospital. J Pathol Bacteriol. 1954 Jul;68(1):55–62. doi: 10.1002/path.1700680107. [DOI] [PubMed] [Google Scholar]
  15. TURCK M., PETERSDORF R. G. The epidemiology of nonenteric Escherichia coli infections: prevalence of serological groups. J Clin Invest. 1962 Sep;41:1760–1765. doi: 10.1172/JCI104635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. UJVARY G. Uber die ätiologische Rolle der Escherichia-coli-Gruppe bei extraenteral lokalisierten Infektionen; biochemische und serologische Untersuchungen. Zentralbl Bakteriol Orig. 1958 Jan;170(6-7):394–405. [PubMed] [Google Scholar]
  17. WAHBA A. H. HOSPITAL INFECTION WITH PSEUDOMONAS PYOCYANEA: AN INVESTIGATION BY A COMBINED PYOCINE AND SEROLOGICAL TYPING METHOD. Br Med J. 1965 Jan 9;1(5427):86–89. doi: 10.1136/bmj.1.5427.86. [DOI] [PMC free article] [PubMed] [Google Scholar]

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