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. 2015 Oct 24;6(33):34143–34157. doi: 10.18632/oncotarget.6219

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Copyright: © 2015 Kim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. HepG2 cells were pretransduced with 100 MOI of FoxO6 vector for 24 hr and then treated with or without LPS (100 ng/ml) for 4 hr. Cells were immunostaining using rabbit anti-NF-κB antibody followed by IgG conjugated with fluorescein isothiocyanate (Red). Bar = 10 μm. B. HepG2 cells were transiently transfected with a NF-κB-containing plasmid linked to the luciferase gene, pre-incubated with FoxO6 (100 MOI) or FoxO6-siRNA (100 MOI) for 24 hr and then treated with LPS for 2 hr. Results are presented in relative luminescence units (RLU). Results were obtained using one-factor ANOVA: ^###p < 0.001 vs. untransduced cells; ^*p < 0.05, ^**p < 0.01, ***p < 0.001 vs. NF-κB-luciferase transduced cells; ^$$p < 0.01 vs. NF-κB-luciferase transduced cells pre-incubated with FoxO6 virus or FoxO6-siRNA. C. HepG2 cells were transiently transfected with a NF-κB-containing plasmid linked to the luciferase gene, pre-incubated with Pak-siRNA (20 nM) for 48 hr and then treated with LPS for 2 hr. Results are presented in relative luminescence units (RLU). Results were obtained using one-factor ANOVA: ^###p < 0.001 vs. untransduced cells; ***p < 0.001 vs. NF-κB-luciferase transduced cells.

A. HepG2 cells were pretransduced with 100 MOI of FoxO6 vector for 24 hr and then treated with or without LPS (100 ng/ml) for 4 hr. Cells were immunostaining using rabbit anti-NF-κB antibody followed by IgG conjugated with fluorescein isothiocyanate (Red). Bar = 10 μm. B. HepG2 cells were transiently transfected with a NF-κB-containing plasmid linked to the luciferase gene, pre-incubated with FoxO6 (100 MOI) or FoxO6-siRNA (100 MOI) for 24 hr and then treated with LPS for 2 hr. Results are presented in relative luminescence units (RLU). Results were obtained using one-factor ANOVA: ^###p < 0.001 vs. untransduced cells; ^*p < 0.05, ^**p < 0.01, ***p < 0.001 vs. NF-κB-luciferase transduced cells; ^$$p < 0.01 vs. NF-κB-luciferase transduced cells pre-incubated with FoxO6 virus or FoxO6-siRNA. C. HepG2 cells were transiently transfected with a NF-κB-containing plasmid linked to the luciferase gene, pre-incubated with Pak-siRNA (20 nM) for 48 hr and then treated with LPS for 2 hr. Results are presented in relative luminescence units (RLU). Results were obtained using one-factor ANOVA: ^###p < 0.001 vs. untransduced cells; ***p < 0.001 vs. NF-κB-luciferase transduced cells.