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. 2015 Oct 5;6(33):34342–34357. doi: 10.18632/oncotarget.5326

Figure 2. Polyubiquitination of Human Numb by LNX2.

Figure 2

In vitro ubiquitination assays were performed for Numb in the presence (+) or absence (−) of each of E1, E2 (UbcH5b), FL-LNX2, GST-Numb, GST, Ubiquitin (Ub) and ATP respectively. Shown are the immunoblots for polyubiquitination of Numb using anti-Numb antibody A. and anti-GST antibody B.