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. 2015 Oct 7;6(33):34592–34605. doi: 10.18632/oncotarget.5423

Figure 6. ILKAP modulated radiation-induced phosphorylation of DNA repair proteins.

Figure 6

A. Protein expression and phosphorylation of indicated proteins after ILKAP knockdown and irradiation (Co, non-specific siRNA control). β-Actin was used as loading control. Fold change was calculated by normalization of densitometric values to the level of the control siRNA cells 15 minutes after irradiation with 6 Gy. B. Basal and radiation survival of A172 and U87MG cells after siRNA mediated downregulation of DNAPK or ATM. Non-specific siRNA was used as control (Co siRNA). Knockdown efficiency is shown by Western blotting. Surviving fraction and radiosensitivity of U87MG cells after combined knockdown of C. ILKAP and DNAPK or D. ILKAP, p53 and DNAPK. Data are mean ± SD (n = 3; t-test; *P < 0.05, **P < 0.01).