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. 2015 Sep 22;6(31):30628–30639. doi: 10.18632/oncotarget.5790

Figure 3. PSD95 expression levels in response to radiation.

Figure 3

A. E18 hippocampal neurons were irradiated with 0, 0.2, or 2 Gy radiation on DIV 7. After 5 days, cell lysates were collected and analyzed by western blotting using anti-PSD95 or actin antibody. Level of PSD95 was normalized to actin. Quantified results are shown as mean ± S.E.M from three independent experiments. B. E18 hippocampal neurons were irradiated with 0 or 0.2 Gy radiation on DIV 7. Five days after radiation, neurons were incubated with FM® 4-64FX dye followed by increasing concentrations of K+ and images were taken. The intensity of fluorescence was quantified as described in material and methods. Blue line: 0 Gy radiation (n = 8) and red line: 0.2 Gy radiation (n = 22).