Figure 5. Autophagy combination therapy suppresses SOC in vivo.
A. C57BL/6 immunocompetent mice were injected IP with 3 × 106 ID8ip-mCherry cells. After two weeks, mice were orally gavaged daily with control, Necramed, or with chloroquine (C) and nelfinavir (N) only. Following two weeks of treatment, mice were euthanized and mCherry fluorescent tumors quantified. B. The weight profiles of mice in (A) Error bars are s.e.m. C. A patient derived xenograft model, using ascites cells from a recurrent platinum-insensitive patient, was established by injecting 3 million PS#3971 cells IP into nude mice. One week following injection, mice were gavaged daily with control or 5%N Necramed (Nelfinavir reduced to 25 mg/kg), for 32 days. Mice were euthanized and macroscopic tumors dissected and weighed. Wilcoxon rank sum test was used to statistically compare groups. D. Immunoblots of pooled tumors dissected from each mouse in (C).