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. 2015 Sep 5;6(31):31216–31232. doi: 10.18632/oncotarget.5157

Figure 5. Apigenin binding to IKKα and IKKβ by ex vivo pull down assay.

Figure 5

A. PC-3 and B. 22Rv1 cells were used for whole cell lysate precipitated with sepharose 4B beads, sepharose 4B-apigenin and sepharose 4B-PS1145 coupled beads. Whole cell lysate (input control, lane 1), precipitate with sepharose 4B beads (negative control, lane 2), sepharose 4B-apigenin coupled beads (lane 3) and sepharose 4B-PS1145 coupled beads were applied to SDS-PAGE, and detected with antibodies against IKKα and IKKβ after transferring the membrane. An increased binding of IKKα was observed in sepharose B-apigenin coupled beads in both cell lines whereas modest binding was observed for IKKβ with apigenin. Details are described in ‘materials and methods’ section.