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. 2015 Sep 8;6(31):31378–31398. doi: 10.18632/oncotarget.5145

Figure 9. Activation of MET signaling in human mesenchymal stem cells induces prolonged survival and expression of myogenic markers.

Figure 9

A. MSC were transduced with viral vectors encoding GFP and TPR-MET and selected with blasticidin. Expression of TPR-MET transgene at mRNA level was verified by PCR. The picture shows one representative experiment. B. Representative photos show morphology of MSC after 3 and 5 passages. After 5 passages control MSC are senescent, whereas TPR-MET MSC still grow. C. When TPR-MET MSC were cultured for several months, expression of myogenic factors, such as PAX3, PAX7, MYF5, MyoD and MRF4, was induced, whereas expression of osteoblastic RUNX2 and adipogenic PPARG2 factors decreases, what coincided with upregulation of SNAI1, qPCR, n = 2. *p < 0.05. Data in graphs are represented as mean +/− SEM.