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. 2015 Sep 10;6(32):33834–33848. doi: 10.18632/oncotarget.5616

Figure 5. Silencing of HIF-1α gene and HIF-1α binding to EWS.

Figure 5

A. Western blot analysis of HIF-1 and EWS-FLI-1 proteins following silencing of HIF-1α in SK-ES1 cell line, resulted in a decrease in HIF-1α and in EWS-FLI-1 protein levels. B. Silencing of HIF-1α led to a decrease in cell growth following 48 hrs using XTT proliferation analysis. C. The migration ability of the cells was measured by Cell Migration assay following silencing of HIF-1α. D. An illustration of 17 HREs 3000bp upstream the start codon and part of the intron of EWS. E. Proteins cross-linked to chromatin from SK-ES1 cells were precipitated with specific antibody directed against HIF-1α and the associated DNA sequences were amplified with PCR primers that amplify the 8 regions. PCR products from input chromatin before- (i- input) and after immunoprecipitation (IP)- (e-elution) are shown.