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A. H&E staining of tumors from MMTV-rtTA:IL24tet-on:MMTV-Her2/neu triple transgenic mice fed regular chow or chow containing doxycycline. Original magnification, 400X. B. Western blot demonstrating lack of expression of MDA-7/IL-24 in tumors that arose in either doxycycline-treated or untreated mice. Each lane contained 50μg protein; the positive control lane contained protein isolated from the mammary glands of double transgenic mice fed doxycyline as shown in Figure 1A.
