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. 2015 Oct 2;6(35):37185–37199. doi: 10.18632/oncotarget.5953

Figure 6. DMBA-treated Cx26 knockout mice exhibit similar incidence of metastases to the lungs.

Figure 6

A., B. Hematoxylin and eosin stained lung sections were evaluated for evidence of lung tumors revealing a similar proportion of mice that developed lung tumors in Cre+ and Cre- mice. C. Evaluation of average lung tumor area per mouse between Cre+ and Cre- mice revealed the likelihood of Cre- mice having larger lung tumor areas compared to Cre+ mice. D. Representative images of paraffin-embedded lung sections immunolabelled for Ki67 (Red), Cx26 (Red) and Cx43 (Red) revealed a similar percentage of lung tissue expressing high levels of Ki67 positivity between Cre- and Cre+ mice and tumors mostly negative, but not always (insert), for Cx26 and Cx43 expression. Hoechst denotes Nuclei. Scale bars = 50 μm. E. Quantification of Ki67, Cx26 and Cx43 immunofluorescent analysis.