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. 2015 Oct 14;6(35):37930–37947. doi: 10.18632/oncotarget.6122

Figure 2. Synergistic interaction between AZD1208 and AZD2014 in AML cells.

Figure 2

Figure 2

Figure 2

Figure 2

Figure 2

Figure 2

Figure 2

A. MOLM-13, MOLM-14, MOLM-16, MV4;11, and OCI-AML3 AML cells were cultured for 72 h in the presence of escalating doses of AZD1208, AZD2014, or the combination at a fixed ratio (10:1, except MOLM-16, 4:1). The AZD1208 concentrations used were 0, 0.5, 1, or 2 μM for MOLM-16 and 0, 2.5, 5, or 10 μM for the other cell lines; the AZD2014 concentrations used were 0, 0.125, 0.25, or 0.5 μM for MOLM-16 and 0, 0.25, 0.5, or 1 μM for the other cell lines. The viable cells were counted by the trypan blue exclusion method and apoptosis was determined by Annexin V staining positivity. Graph shows the mean ± SEM of results of three independent experiments B. Cells derived from 6 primary AML samples were treated with AZD1208, AZD2014, or the combination in various concentrations in the presence of MSCs and tested for viability and cell death after 24 h by FACS. Patients #2-4 have FLT3-ITD mutation-positive disease. C. Clonogenic assay of cells derived from primary AML samples 14 days after AZD1208, AZD2014, or combination treatment. Patient #10 has FLT3-ITD mutation-positive disease.