Figure 2. Increased recruitment of septins to microtubules confers Taxol^® resistance.

A. Patterns of septin whole cell expression (Lysates) and recruitment to MTs in Tr vs Ts cells. A constant protein (20 μg for Lysates) or α-tubulin amount (for MT fractions) was loaded on gels for each sample. B. SEPT2 localization switches from F-actin in Ts to MTs in Tr cells. Scale bar = 10 μm. C. Knockdown of individual septins affects the presence of other septins in the MT fraction of Tr cells. D. Individual septin inhibition alters the formation of septin filaments and their localization to MTs in Tr cells. E. Individual septin inhibition by RNAi restores chemosensitivity of Tr cells to Taxol^® as determined by MTT assay. F. SEPT2 depletion (72 h RNAi) did not cause the accumulation of Tr cells in G2/M.