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. 1993 Sep 15;90(18):8702–8706. doi: 10.1073/pnas.90.18.8702

A model for tumor suppression using H-1 parvovirus.

A Telerman 1, M Tuynder 1, T Dupressoir 1, B Robaye 1, F Sigaux 1, E Shaulian 1, M Oren 1, J Rommelaere 1, R Amson 1
PMCID: PMC47426  PMID: 8378352

Abstract

A model system is proposed to investigate, at the molecular level, the pathways of tumor suppression. As a tool for the selection of cells with a suppressed phenotype, we used the H-1 parvovirus that preferentially kills various neoplastic cells. From the human K562 leukemia cells, we isolated a clone, KS, that is resistant to the cytopathic effect of the H-1 virus and displays a suppressed malignant phenotype. The suppressed malignancy and the cellular resistance to H-1 killing appear to depend on the activity of wild-type p53. Whereas the KS cells express wild-type p53, the protein is undetectable in the parental K562 cells. Experiments with p53 mutants suggest that wild-type p53, in its functionally intact state, contributes to the resistance against the cytopathic effect of H-1 parvovirus.

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