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. 1969 Nov;22(6):634–641. doi: 10.1136/jcp.22.6.634

Gram-negative bacilli in burns

Brenda Davis 1, H A Lilly 1, E J L Lowbury 1
PMCID: PMC474329  PMID: 4312399

Abstract

In a period of two years, 865 strains of Gram-negative bacilli other than Pseudomonas aeruginosa isolated from burns were identified by a range of tests. The commonest species were Proteus mirabilis, Escherichia coli, and Enterobacter cloacae. Many strains of Klebsiella aerogenes, Enterobacter aerogenes, and Bacterium anitratum were also found.

A large proportion of the strains were tested for sensitivity to nalidixic acid, ampicillin, kanamycin, chloramphenicol, tetracycline, and carbenicillin, and smaller numbers of strains were tested for sensitivity to cephaloridine, polymyxin, streptomycin, sulphadiazine, sulfamylon, and trimethoprim. The proportion of strains sensitive and resistant to different antibacterial agents varied widely with species of bacteria. A large proportion of the strains of E. coli and P. mirabilis were resistant to ampicillin, which was much used in treatment; resistance appeared least often towards nalidixic acid, kanamycin, trimethoprim, and gentamicin. Multiple resistance occurred less often among strains of E. coli than among Klebsiella spp, Enterobacter spp, and P. mirabilis.

Phage and serological typing of Ps. aeruginosa showed that most infections of burns with this organism were due to strains previously found in other patients in the same ward. Taken with other evidence, this supported the view that most infections with Ps. aeruginosa were not acquired from the patient's own flora but from sources in the hospital environment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARBER M., WATERWORTH P. M. PENICILLINASE-RESISTANT PENICILLINS AND CEPHALOSPORINS. Br Med J. 1964 Aug 8;2(5405):344–349. doi: 10.1136/bmj.2.5405.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BAZAN A., CHAVEZ P. M., GURMENDI G., MARKLEY K. Fatal Pseudomonas septicemias in burned patients. Ann Surg. 1957 Feb;145(2):175–181. doi: 10.1097/00000658-195702000-00005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BUSHBY S. R. Sensitivity tests for the polymyxins. J Clin Pathol. 1955 May;8(2):120–122. doi: 10.1136/jcp.8.2.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown V. I., Lowbury E. J. Use of an improved cetrimide agar medium and other culture methods for Pseudomonas aeruginosa. J Clin Pathol. 1965 Nov;18(6):752–756. doi: 10.1136/jcp.18.6.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. COWAN S. T., STEEL K. J. Diagnostic tables for the common medical bacteria. J Hyg (Lond) 1961 Sep;59:357–372. doi: 10.1017/s0022172400039024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cason J. S., Jackson D. M., Lowbury E. J., Ricketts C. R. Antiseptic and aseptic prophylaxis for burns: use of silver nitrate and of isolators. Br Med J. 1966 Nov 26;2(5525):1288–1294. doi: 10.1136/bmj.2.5525.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cason J. S., Lowbury E. J. Mortality and infection in extensively burned patients treated with silver-nitrate compresses. Lancet. 1968 Mar 30;1(7544):651–654. doi: 10.1016/s0140-6736(68)92095-3. [DOI] [PubMed] [Google Scholar]
  8. Datta N. Drug resistance and R factors in the bowel bacteria of London patients before and after admission to hospital. Br Med J. 1969 May 17;2(5654):407–411. doi: 10.1136/bmj.2.5654.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FINLAND M. Treatment of pneumonia and other serious infections. N Engl J Med. 1960 Aug 4;263:207–221. doi: 10.1056/NEJM196008042630501. [DOI] [PubMed] [Google Scholar]
  10. Fox C. L., Jr Silver sulfadiazine--a new topical therapy for Pseudomonas in burns. Therapy of Pseudomonas infection in burns. Arch Surg. 1968 Feb;96(2):184–188. doi: 10.1001/archsurg.1968.01330200022004. [DOI] [PubMed] [Google Scholar]
  11. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones R. J., Jackson D. M., Lowbury E. J. Antiserum and antibiotic in the prophylaxis of burns against Pseudomonas aeruginosa. Br J Plast Surg. 1966 Jan;19(1):43–57. doi: 10.1016/s0007-1226(66)80007-3. [DOI] [PubMed] [Google Scholar]
  13. KOHN J. A preliminary report of a new gelatin liquefaction method. J Clin Pathol. 1953 Aug;6(3):249–249. doi: 10.1136/jcp.6.3.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KOVACS N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature. 1956 Sep 29;178(4535):703–703. doi: 10.1038/178703a0. [DOI] [PubMed] [Google Scholar]
  15. LIEDBERG N. C. F., REISS E., KUHN L. R., AMSPACHER W. H., ARTZ C. P. Infection in burns. IV. Evaluation of the local use of chloramphenicol ointment and furacin soluble dressing on granulating surfaces following extensive full thickness burns. Surg Gynecol Obstet. 1955 Feb;100(2):219–231. [PubMed] [Google Scholar]
  16. LISTON J., WIEBE W., COLWELL R. R. QUANTITATIVE APPROACH TO THE STUDY OF BACTERIAL SPECIES. J Bacteriol. 1963 May;85:1061–1070. doi: 10.1128/jb.85.5.1061-1070.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LOWBURY E. J., FOX J. The epidemiology of infection with Pseudomonas pyocyanea in a burns unit. J Hyg (Lond) 1954 Sep;52(3):403–416. doi: 10.1017/s0022172400027601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LOWBURY E. J. Infection of burns. Br Med J. 1960 Apr 2;1(5178):994–1001. doi: 10.1136/bmj.1.5178.994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LOWBURY E. J., LILLY H. A., WILKINS M. D. A cabinet for the detection of fluorescent bacterial cultures. J Clin Pathol. 1962 Jul;15:339–342. doi: 10.1136/jcp.15.4.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Langohr J. L., Owen C. R., Cope O. Bacteriologic Study of Burn Wounds : A Comparison of the Bacterial Flora of Burn Wounds of Patients Treated with Sulfonamides or Penicillin. Ann Surg. 1947 Apr;125(4):452–504. [PMC free article] [PubMed] [Google Scholar]
  21. Lindberg R. B., Moncrief J. A., Switzer W. E., Order S. E., Mills W., Jr The successful control of burn wound sepsis. J Trauma. 1965 Sep;5(5):601–616. doi: 10.1097/00005373-196509000-00004. [DOI] [PubMed] [Google Scholar]
  22. Moorhouse E. C. Transferable drug resistance in enterobacteria isolated from urban infants. Br Med J. 1969 May 17;2(5654):405–407. doi: 10.1136/bmj.2.5654.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. ROGERS K. B., TAYLOR J. Laboratory diagnosis of gastroenteritis due to Escherichia coli. Bull World Health Organ. 1961;24:59–71. [PMC free article] [PubMed] [Google Scholar]
  24. SHAW C., CLARKE P. H. Biochemical classification of Proteus and Providence cultures. J Gen Microbiol. 1955 Aug;13(1):155–161. doi: 10.1099/00221287-13-1-155. [DOI] [PubMed] [Google Scholar]
  25. Sachs A., Watson J. Four years' experience at a specialised burns centre. The McIndoe Burns Centre 1965-68. Lancet. 1969 Apr 5;1(7597):718–721. doi: 10.1016/s0140-6736(69)92663-4. [DOI] [PubMed] [Google Scholar]
  26. Shooter R. A., Walker K. A., Williams V. R., Horgan G. M., Parker M. T., Asheshov E. H., Bullimore J. F. Faecal carriage of Pseudomonas aeruginosa in hospital patients. Possible spread from patient to patient. Lancet. 1966 Dec 17;2(7477):1331–1334. doi: 10.1016/s0140-6736(66)92082-4. [DOI] [PubMed] [Google Scholar]
  27. TALBOT J. M., CUNLIFFE A. C., GOWER N. D. The differentiation of coliform organisms infecting the urinary tract. J Clin Pathol. 1957 Aug;10(3):222–225. doi: 10.1136/jcp.10.3.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. TOPLEY E., LOWBURY E. J. L., HURST L. Bacteriological control of aureomycin therapy. Lancet. 1951 Jan 13;1(6646):87–89. doi: 10.1016/s0140-6736(51)91169-5. [DOI] [PubMed] [Google Scholar]
  29. WAHBA A. H. HOSPITAL INFECTION WITH PSEUDOMONAS PYOCYANEA: AN INVESTIGATION BY A COMBINED PYOCINE AND SEROLOGICAL TYPING METHOD. Br Med J. 1965 Jan 9;1(5427):86–89. doi: 10.1136/bmj.1.5427.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Watt P. J., Okubadejo O. A. Changes in incidence and aetiology of bacteraemia arising in hospital practice. Br Med J. 1967 Jan 28;1(5534):210–211. doi: 10.1136/bmj.1.5534.210. [DOI] [PMC free article] [PubMed] [Google Scholar]

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