Abstract
Fine needle aspiration biopsy smears from 19 patients with porphyria cutanea tarda were studied by fluorescence microscopy. Fluorescence was arbitrarily graded from 0 to 4. All patients—those with clinically manifest as well as those with latent disease—showed bright red fluorescence of porphyrin type in the liver cells, though in latent cases the fluorescence commonly was of a low grade. In patients with manifest disease fluorescence of grade 3 or 4 was present.
In patients with fluorescence of grades 1 or 2 the uroporphyrin excretion in urine was normal or slightly increased. At higher grades all levels of porphyrin excretion were encountered.
Of 22 control subjects, only one showed hepatic fluorescence of a porphyrin character. The control patient with a positive finding was found to have definitely increased urinary uroporphyrin excretion and probably has latent porphyria cutanea tarda.
The procedure described should be useful as a screening test for hepatic cutaneous porphyria.
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- ASKEVOLD R. Routine analysis of porphyrines in urine. Scand J Clin Lab Invest. 1951;3(4):318–319. doi: 10.3109/00365515109060622. [DOI] [PubMed] [Google Scholar]
- FRANZEN S., GIERTZ G., ZAJICEK J. Cytological diagnosis of prostatic tumours by transrectal aspiration biopsy: a preliminary report. Br J Urol. 1960 Jun;32:193–196. doi: 10.1111/j.1464-410x.1960.tb03763.x. [DOI] [PubMed] [Google Scholar]
- HOLTI G., RIMINGTON C., TATE B. C., THOMAS G. An investigation of porphyria cutanea tarda. Q J Med. 1958 Jan;27(105):1–18. [PubMed] [Google Scholar]
- Hickman R., Saunders S. J., Eales L. Treatment of symptomatic porphyria by venesection. S Afr Med J. 1967 May 6;41(18):456–460. [PubMed] [Google Scholar]
- IPPEN H. [Pathogenesis and therapy of porphyria cutanea tarda (chronic hepatic porphyria). Preliminary report]. Klin Wochenschr. 1960 Jan 15;38:89–89. doi: 10.1007/BF01483617. [DOI] [PubMed] [Google Scholar]
- LAMONT N. M., HATHORN M., JOUBERT S. M. Porphyria in the African: a study of 100 cases. Q J Med. 1961 Oct;30:373–392. [PubMed] [Google Scholar]
- Lundvall O., Weinfeld A. Studies of the clinical and metabolic effects of phlebotomy treatment in porphyria cutanea tarda. Acta Med Scand. 1968 Sep;184(3):191–199. doi: 10.1111/j.0954-6820.1968.tb02443.x. [DOI] [PubMed] [Google Scholar]
- MAUZERALL D., GRANICK S. The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine. J Biol Chem. 1956 Mar;219(1):435–446. [PubMed] [Google Scholar]
- RIMINGTON C., SVEINSSON S. L. The spectrophotometric determination of uroporphyrin. Scand J Clin Lab Invest. 1950;2(3):209–216. doi: 10.3109/00365515009049872. [DOI] [PubMed] [Google Scholar]
- SCHMID R., SCHWARTZ S., WATSON C. J. Porphyrin content of bone marrow and liver in the various forms of porphyria. AMA Arch Intern Med. 1954 Feb;93(2):167–190. doi: 10.1001/archinte.1954.00240260001001. [DOI] [PubMed] [Google Scholar]
- UYS C. J., EALES L. THE HISTOPATHOLOGY OF THE LIVER IN ACQUIRED (SYMPTOMATIC) PORPHYRIA. S Afr J Lab Clin Med. 1963 Dec;14:190–197. [PubMed] [Google Scholar]
- WATSON C. J., PIMENTA de MELLO R., SCHWARTZ S., HAWKINSON V. E., BOSSENMAIER I. Porphyrin chromogens or precursors in urine, blood, bile, and feces. J Lab Clin Med. 1951 Jun;37(6):831–842. [PubMed] [Google Scholar]
- WITH T. K. Porphyrin concentration from ultraviolet extinction; a note on the calculation. Scand J Clin Lab Invest. 1955;7(2):193–194. doi: 10.3109/00365515509134507. [DOI] [PubMed] [Google Scholar]