The authors would like to correct Fig 2A and Fig 4. In Fig 2A, while preparing the figure for publication, the same image was erroneously used for the Lycop 5 μM plate and the beta-carot 10μM plate. The authors have provided a corrected version of Fig 2, which includes the correct image for the Lycop 5 μM plate. In Fig 4, while preparing the figure for publication, the same image was erroneously used for the Lycop 5μM flow cytometry plot and the beta-carot 5μM plot. The authors have provided a corrected version of Fig 4, which includes the correct image for the beta-carot 5μM plot. The underlying data and unedited images for Fig 2A and Fig 4 are available as Supporting Information. The authors confirm that these errors do not alter their results.
Fig 2. Formation of AtT-20 colonies.
The number of AtT-20 colonies was determined after 21 days of culture in DMEM supplemented with 10% FCS containing lycopene (Lycop) and beta-carotene (beta-carot) at concentrations of 5 and 10 μM. The number of colonies formed was detected by crystal violet staining. Phase contrast microscopy of AtT-20 cell colonies was observed on 6-well culture plates (A) and quantitative representation of the colonies formed (B). Data are presented as mean±standard deviation of 3 independent experiments, each performed at least in duplicate. *indicates significant differences from control group (**p<0.01, ***p<0.001).
Fig 4. Detection of apoptotic AtT-20 cells by flow cytometry under lycopene or beta-carotene stimulation at the concentrations of 5 and 10 μM for 96 h.
When the cells were treated with lycopene and carotene, the apoptosis rate increased significantly at concentrations of 5 and 10 μM. Beta-carotene at 10 μM induced a greater increase in the rate of apoptosis compared with the other experimental conditions. Data are expressed as mean±standard deviation relative to the control, of 3 independent experiments, each performed with at least 3 replicates. *indicates significant differences from control group (*p<0.05, **p<0.01, ***p<0.001).
Supporting Information
Reference
- 1.Haddad NF, Teodoro AJ, Leite de Oliveira F, Soares N, de Mattos RM, Hecht F, et al. (2013) Lycopene and Beta-Carotene Induce Growth Inhibition and Proapoptotic Effects on ACTH-Secreting Pituitary Adenoma Cells. PLoS ONE 8(5): e62773 doi:10.1371/journal.pone.0062773 [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.