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. 1970 Feb;23(1):47–53. doi: 10.1136/jcp.23.1.47

An automated method for the measurement of folate activity

R E Davis 1, D J Nicol 1, A Kelly 1
PMCID: PMC474411  PMID: 5439088

Abstract

A new approach to the estimation of folate activity is described. The system may be used as a semi-automated method or as a fully automated method using the AutoAnalyzer or Mecolab equipment. The methods use a chloramphenicol-resistant strain of L. casei as the test organism, and this eliminates the need for sterilization or aseptic addition. The systems are able to handle extremely large numbers of samples in a relatively short time. Results are similar to those obtained by standard techniques.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER H., HERBERT V., FRANK O., PASHER I., HUTNER S. H., WASSERMAN L. R., SOBOTKA H. A microbiologic method for detecting folic acid deficiency in man. Clin Chem. 1959 Aug;5(4):275–280. [PubMed] [Google Scholar]
  2. Cooperman J. M. Microbiological assay of serum and whole-blood folic acid activity. Am J Clin Nutr. 1967 Sep;20(9):1015–1024. doi: 10.1093/ajcn/20.9.1015. [DOI] [PubMed] [Google Scholar]
  3. DAVIS R. E., KELLY A. The microbiological assay of serum folic acid activity. Aust J Exp Biol Med Sci. 1962 Dec;40:437–444. doi: 10.1038/icb.1962.48. [DOI] [PubMed] [Google Scholar]
  4. FROST D. V., LAPIDUS M., PLAUT K. A., SCHERFLING E., FRICKE H. H. Differential stability of various analogs of cobalamin to vitamin C. Science. 1952 Aug 1;116(3005):119–121. doi: 10.1126/science.116.3005.119. [DOI] [PubMed] [Google Scholar]
  5. HERBERT V. The assay and nature of folic acid activity in human serum. J Clin Invest. 1961 Jan;40:81–91. doi: 10.1172/JCI104240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harper T. A. A modified 'aseptic addition' assay procedure for the measurement of serum 'folic-acid' activity. Nature. 1965 Aug 28;207(5000):947–949. doi: 10.1038/207947a0. [DOI] [PubMed] [Google Scholar]
  7. Herbert V. Aseptic addition method for Lactobacillus casei assay of folate activity in human serum. J Clin Pathol. 1966 Jan;19(1):12–16. doi: 10.1136/jcp.19.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoffbrand A. V., Newcombe F. A., Mollin D. L. Method of assay of red cell folate activity and the value of the assay as a test for folate deficiency. J Clin Pathol. 1966 Jan;19(1):17–28. doi: 10.1136/jcp.19.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. WATERS A. H., MOLLIN D. L. Observations on the metabolism of folic acid in pernicious anaemia. Br J Haematol. 1963 Jul;9:319–327. doi: 10.1111/j.1365-2141.1963.tb06556.x. [DOI] [PubMed] [Google Scholar]

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