Video 1. Optical tweezers manipulate a single SNARE complex to reveal its folding intermediates, energetics, and kinetics.
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DOI: 10.7554/eLife.09580.009
Not drawn to scale. Note that the single-SNARE complex was being pulled or relaxed by moving the optical trap on the right (not shown) at a speed of 10 nm/s. The tension and extension of the DNA-protein tether was monitored to derive the conformations, energetics, and kinetics of SNARE assembly.